Journal of Biosciences

, Volume 3, Issue 2, pp 149–155

Cardiotoxin of the Indian cobra(Naja naja) is a pyrophosphatase

  • K. E. Achyuthan
  • L. K. Ramachandran

DOI: 10.1007/BF02702658

Cite this article as:
Achyuthan, K.E. & Ramachandran, L.K. J Biosci (1981) 3: 149. doi:10.1007/BF02702658


An inorganic pyrophosphatase has been purified to apparent homogeniety from Indian cobra(Naja naja) venom, with a ten-fold increase in specific activity. The enzyme activity is intrinsic to a protein fraction in the venom which is normally termed cardiotoxin, cobramine, cytotoxin and so on. The enzyme shows a lowKm (70 μI) and high heat stability. The enzyme was active against sodium pyrophosphate; it also hydrolyses a few mononucletides and sugar phosphates at much lower rates. The physiological significance of inorganic pyrophosphatase in venom is discussed.


Cobra venomNaja najacardiotoxinpyrophosphatase

Copyright information

© Indian Academy of Sciences 1981

Authors and Affiliations

  • K. E. Achyuthan
    • 1
  • L. K. Ramachandran
    • 1
  1. 1.Department of BiochemistryOsmania UniversityHyderabad