Chromatographia

, Volume 24, Issue 1, pp 827–831

Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection

  • G. Damsma
  • D. Lammerts van Bueren
  • B. H. C. Westerink
  • A. S. Horn
Article

DOI: 10.1007/BF02688592

Cite this article as:
Damsma, G., van Bueren, D.L., Westerink, B.H.C. et al. Chromatographia (1987) 24: 827. doi:10.1007/BF02688592

Summary

The measurement of choline and acetylcholine by means of HPLC, a post-column enzyme reactor, and electrochemical detection has been simplified and optimised. The use of a cation exchanger and enzyme reactor fitted in a cartridge holder appeared to result in reproducible, sensitive, and selective measurement of endogenous choline and acetylcholine with a lower detection limit of 50 fmole.

Key Words

Column liquid chromatographyAcetylcholineCholineEnzyme reactorElectrochemical detection

Copyright information

© Friedr. Vieweg & Sohn Verlagsgeseilschaft mbH 1987

Authors and Affiliations

  • G. Damsma
    • 1
  • D. Lammerts van Bueren
    • 2
  • B. H. C. Westerink
    • 1
  • A. S. Horn
    • 1
  1. 1.Department of Medicinal ChemistryUniversity of GroningenGroningenThe Netherlands
  2. 2.Chrompack InternationalMiddelburgThe Netherlands