Molecular Biotechnology

, Volume 35, Issue 2, pp 185–197

Methods and goals for the use of in vitro and in vivo chemosensitivity testing

Authors

    • Garden State Cancer Center
  • David M. Goldenberg
    • Garden State Cancer Center
Review

DOI: 10.1007/BF02686104

Cite this article as:
Blumenthal, R.D. & Goldenberg, D.M. Mol Biotechnol (2007) 35: 185. doi:10.1007/BF02686104

Abstract

Sensitive, specific, and accurate methods to assay chemosensitivity are needed to (1) screen new therapeutic agents, (2) identify patterns of chemosensitivity for different tumor types, (3) establish patterns of cross-resistance and sensitivity in treatment of naïve and relapsing tumors, (4) identify genomic and proteomic profiles associated with sensivity, (5) correlate in vitro response with preclinical in vivo effects and clinical outcomes for a particular therapeutic agent, and (6) tailor chemotherapy regimens to individual patients. Various methods are available to achieve these end points, including several in vitro clonogenic and proliferation assays, cell metabolic activity assays, molecular assay to monitor expression of markers for responsiveness, drug resistance, and for induction of apoptosis, in vivo tumor growth and survival assays in metastatic and orthotopic models, and in vivo imaging assays. The advantages and disadvantages of the specific assays are discussed. A summary of research questions related to chemosensitivity testing is also included.

Index Entries

Dose-response curve IC50 values imaging metabolic assays molecular markers proliferation assays

Copyright information

© Humana Press Inc 2007