Biological Trace Element Research

, Volume 115, Issue 2, pp 195–202

Study on the toxic effect of lead(II) ion on Escherichia coli

  • Liu Peng
  • Ruan Lifang
  • Xiao Hongyu
  • Li Xi
  • Zhang Chaocan
Article

DOI: 10.1007/BF02686030

Cite this article as:
Peng, L., Lifang, R., Hongyu, X. et al. Biol Trace Elem Res (2007) 115: 195. doi:10.1007/BF02686030

Abstract

The toxic effects of lead(II) have been studied in Escherichia coli cells. Using microcalorimetric analysis, it was shown that E. coli growth was inhibited in the presence of Pb2+ resulting from damage to the cell membrane and that Pb2+ takes part in the metabolism of cells. Treatment with lysozyme confirms damage to the cell’s outer membrane. Similarities between the ionic radii and charge/radius ratio cause Pb(II) to replace Ca(II) at the binding sites of lipopolysacharides, leading to rupture of protecting areas on the cell’s surface. Consequently, the protection and functionality of outer membrane is lost, thus becoming the basis for the biological effect of Pb2+ on E. coli.

Index Entries

Leadtoxic effectmicrocalorimetryinhibitionouter membrane

Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Liu Peng
    • 1
  • Ruan Lifang
    • 3
  • Xiao Hongyu
    • 1
  • Li Xi
    • 1
  • Zhang Chaocan
    • 2
  1. 1.School of SciencesWuhan University of TechnologyWuhanChina
  2. 2.School of Material Science and TechnologyWuhan University of TechnologyWuhanChina
  3. 3.State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhanChina