Molecular Biotechnology

, 35:215

Expression of a synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat labile enterotoxin in rice endosperm

  • Maria Oszvald
  • Tae-Jin Kang
  • Sandor Tomoskozi
  • Cecilia Tamas
  • Laszlo Tamas
  • Tae-Geum Kim
  • Moon-Sik Yang
Research

DOI: 10.1007/BF02686007

Cite this article as:
Oszvald, M., Kang, TJ., Tomoskozi, S. et al. Mol Biotechnol (2007) 35: 215. doi:10.1007/BF02686007

Abstract

Epitopes often require co-delivery with adjuvant and targeting proteins to enable recognition by the immune system, and this approach may also increase the efficacy of the antigen. In this study, we assess and describe the ability of transgenic rice plants to express a fusion protein consisting of the B-subunit of the Escherichia coli heat-labile enterotoxin (LTB) and a synthetic core-neutralizing epitope (COE) of porcine epidemic diarrhea virus (PEDV), inducing an enteric disease that is seen most predominantly in piglets. Both components of the fusion proteins were detected with Western blot analysis. The fusion protein was determined to assemble into pentamers, as was evidenced by its ability to bind to GM1 gangliosides, and evidenced an average level of expression in a trangenic rice endosperm. This indicates that the expression system of the plant is capable of generating a sizable amount of antigen, possibly allowing for the successful development of an edible vaccine.

Index Entries

Edible vaccineEscherichia coli heat labile toxinfusion proteinendosperm-specific expressionporcine epidemic diarrhea virus

Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Maria Oszvald
    • 1
  • Tae-Jin Kang
    • 2
  • Sandor Tomoskozi
    • 1
  • Cecilia Tamas
    • 3
  • Laszlo Tamas
    • 4
  • Tae-Geum Kim
    • 5
  • Moon-Sik Yang
    • 5
  1. 1.Department of Biochemistry and Food TechnologyBudapest University of Technology and EconomicsBudapestHungary
  2. 2.Team of Research and DevelopmentJeonbuk Bioindustry Development InstituteJeonjuSouth Korea
  3. 3.Agricultural Research Institute of the Hungarian Academy of SciencesMartonvasarHungary
  4. 4.Department of Plant PhysiologyEotvos Lorand UniversityBudapestHungary
  5. 5.Division of Biological Science and the Research Center for Bioactive MaterialsChonbuk National UniversityJeonjuSouth Korea