Nuclear DNA content of some important plant species
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Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.
- Arumuganathan, K. and E.D. Earle 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Reporter 9:221–231.
- Bennett, M.D. 1985. Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In:Plant Genetics (ed. M. Freeling), pp. 283–302. Alan R. Liss, New York.
- Bennett, M.D. and J.B. Smith. 1976. Nuclear DNA amounts in angiosperms. Phil. Trans. R. Soc. Lond. B 274:227–274. CrossRef
- Bennett, M.D., J.B. Smith and J.S. Heslop-Harrison. 1982. Nuclear DNA amounts in angiosperms. Proc. R. Soc. Lond. B 216:179–199. CrossRef
- Cameron, M.J. 1990. Macintosh graphics for the EPICS flow cytometer user. Cytometry 11:916–918. CrossRef
- De Rocher, E.J., K.R. Harkins, D.W. Galbraith and H.J. Bohnert. 1990. Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science 250:99–101. CrossRef
- Galbraith, D.W., K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051. CrossRef
- Galbraith, D.W. 1990. Flow cytometric analysis of plant genomes. In:Methods in Cell Biology (eds. Z. Datzynkiewicz and H. Crissman), pp. 549–563. Academic Press, New York.
- Iyengar, G.A.S. and S.K. Sen. 1978. Nuclear DNA content of several wild and cultivatedOryza species. Environ. Exp. Bot. 18:219–224. CrossRef
- Leutwiler, L.S., B.R. Hough-Evans and E.M. Meyerowitz. 1984. The DNA ofArabidopsis thaliana. Mol. Gen. Genet. 194:15–23. CrossRef
- Laurie, D.A. and M.D. Bennett. 1985. Nuclear DNA content in the generaZea andSorghum. Intergeneric, interspecific and intraspecific variation. Heredity 55:307–313.
- Messeguer, R., M.W. Ganal, J.C. Steffens and S.D. Tanksley. 1991. Characterization of the level, target sites, and inheritance of cytosine methylation in tomato nuclear DNA. Plant Mol. Biol. 16:753–770. CrossRef
- Michaelson, M.J., H.J. Price, J.R. Ellison and J.S. Johnston. 1991. Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry. Amer. J. Bot. 78:183–188. CrossRef
- Nuclear DNA content of some important plant species
Plant Molecular Biology Reporter
Volume 9, Issue 3 , pp 208-218
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- Kluwer Academic Publishers
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- Genome size
- flow cytometry
- propidium iodide
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