Plant Molecular Biology Reporter

, Volume 9, Issue 4, pp 294–307

DNA amplification fingerprinting: A strategy for genome analysis

  • Gustavo Caetano-Anollés
  • Brant J. Bassam
  • Peter M. Gresshoff
Review

DOI: 10.1007/BF02672006

Cite this article as:
Caetano-Anollés, G., Bassam, B.J. & Gresshoff, P.M. Plant Mol Biol Rep (1991) 9: 294. doi:10.1007/BF02672006

Abstract

A novel strategy to detect genetic differences among organisms, DNA amplification fingerprinting (DAF), uses a thermostable DNA polymerase directed by usually one short (≥5 bp) oligonucleotide primer of arbitrary sequence to amplify short segments of genomic DNA and generate a range of DNA extension products. These products can be analyzed by polyacrylamide gel electrophoresis and silver staining. DAF is rapid and sensitive and is independent of cloning and prior genetic characterization. Here we describe this new methodology, its application to plant genotyping, and its perspectives in DNA fingerprinting and genome mapping.

Key Words

DNA fingerprinting arbitrary primers PCR genome mapping 

Abbreviations

DAF

DNA amplification fingerprinting

AFLP

amplification fragment length polymorphism

PCR

polymerase chain reaction

cM

centimorgan

STS

sequence-tagged sites

IRS

interspersed repetitive sequences

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Gustavo Caetano-Anollés
    • 1
  • Brant J. Bassam
    • 1
  • Peter M. Gresshoff
    • 1
  1. 1.Plant Molecular Genetics, Institute of Agriculture and Center for Legume ResearchThe University of TennesseeKnoxvilleUSA

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