Plant Molecular Biology Reporter

, Volume 14, Issue 1, pp 17–22

Inhibition of random amplified polymorphic DNAs (RAPDs) by plant polysaccharides

Authors

  • Ram Naresh Pandey
    • Plant Biotechnology CenterBaylor University
  • Robert P. Adams
    • Plant Biotechnology CenterBaylor University
  • Lori E. Flournoy
    • Plant Biotechnology CenterBaylor University
Commentary

DOI: 10.1007/BF02671898

Cite this article as:
Pandey, R.N., Adams, R.P. & Flournoy, L.E. Plant Mol Biol Rep (1996) 14: 17. doi:10.1007/BF02671898

Abstract

A survey of the inhibition of the amplification of spinach DNA by various plant polysaccharides revealed that neutral polysaccharides (arabinogalactan, dextran, gum guar, gum locust bean, inulin, mannan, and starch) were not inhibitory. In contrast, the acidic polysaccharides (carrageenan, dextran sulfate, gum ghatti, gum karaya, pectin, and xylan)were inhibitory. In the process of preparing random amplified polymorphic DNAs (RAPDs), the loss of large DNA bands appears to be an indicator that the fingerprint pattern has been affected by polysaccharides. The addition of various concentrations of Tween 20, DMSO, or PEG 400 to the PCR reaction mixture resulted in partial restoration of amplification of RAPDs for the acidic polysaccharides. The most effective way to eliminate the effects of polysaccharide inhibition was by diluting the DNA extracts, and thereby diluting the polysaccharide inhibitors.

Key Words

RAPDsPCRpolysaccharidesinhibitionplantsbuffer enhancers

Abbreviations

RAPD

random amplified polymorphic DNA

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Copyright information

© International Society for Plant Molecular Biology 1996