Plant Molecular Biology Reporter

, Volume 11, Issue 2, pp 117–121

A procedure for mini-preparations of genomic DNA from needles of silver fir (Abies alba mill.)

  • B. Ziegenhagen
  • P. Guillemaut
  • F. Scholz
Commentary

DOI: 10.1007/BF02670469

Cite this article as:
Ziegenhagen, B., Guillemaut, P. & Scholz, F. Plant Mol Biol Rep (1993) 11: 117. doi:10.1007/BF02670469

Abstract

We have adapted a procedure for the isolation of genomic DNA from needles of silver fir (Abies alba) to meet the requirements for large-scale analysis of the population genetics of forest trees. Our modifications permit the entire procedure to be carried out in Eppendorf tubes, which greatly minimizes time, plant material, and the amounts of chemicals. DNA is recovered with a mean efficiency of 80 μg/g needles, is suitable for restriction by the common endonucleases, and serves as a substrate for PCR.

Key words

DNA minipreparation Abies alba needles 

Copyright information

© International Society for Plant Molecular Biology 1993

Authors and Affiliations

  • B. Ziegenhagen
    • 1
  • P. Guillemaut
    • 2
  • F. Scholz
    • 1
  1. 1.Bundesforschungsanstalt für Forst- und HolzwirtschaftInstitut für ForstgenetikGroßhansdorf 2Germany
  2. 2.Laboratoire de Biologie Végétale AppliquéeInstitut Universitaire de Technologie Louis PasteurStrasbourgFrance

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