Isolation of plant DNA: A fast, inexpensive, and reliable method Authors
Cite this article as: Guillemaut, P. & Maréchal-Drouard, L. Plant Mol Biol Rep (1992) 10: 60. doi:10.1007/BF02669265 Abstract
We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees, herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or detection of adducts.
Key Words DNA isolation woody plant seed cell culture References
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© Kluwer Academic Publishers 1992