Plant Molecular Biology Reporter

, Volume 10, Issue 1, pp 60-65

First online:

Isolation of plant DNA: A fast, inexpensive, and reliable method

  • Pierre GuillemautAffiliated withLaboratoire de Biologie Végétale Appliquée, Institut Universitaire de Technologie Louis Pasteur
  • , Laurence Maréchal-DrouardAffiliated withInstitut de Biologie Moléculaire des Plantes du CNRS

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We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees, herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or detection of adducts.

Key Words

DNA isolation woody plant seed cell culture