Plant Molecular Biology Reporter

, Volume 8, Issue 1, pp 40–49

Medicago truncatula, a model plant for studying the molecular genetics of theRhizobium-legume symbiosis

  • David G. Barker
  • Sylvie Bianchi
  • François Blondon
  • Yvette Dattée
  • Gérard Duc
  • Sadi Essad
  • Pascal Flament
  • Philippe Gallusci
  • Gérard Génier
  • Pierre Guy
  • Xavier Muel
  • Jacques Tourneur
  • Jean Dénarié
  • Thierry Huguet
Genetic Resource

DOI: 10.1007/BF02668879

Cite this article as:
Barker, D.G., Bianchi, S., Blondon, F. et al. Plant Mol Biol Rep (1990) 8: 40. doi:10.1007/BF02668879

Abstract

Medicago truncatula has all the characteristics required for a concerted analysis of nitrogen-fixing symbiosis withRhizobium using the tools of molecular biology, cellular biology and genetics.M. truncatula is a diploid and autogamous plant has a relatively small genome, and preliminary molecular analysis suggests that allelic heterozygosity is minimal compared with the cross-fertilising tetraploid alfalfa (Medicago sativa). TheM. truncatula cultivar Jemalong is nodulated by theRhizobium meliloti strain 2011, which has already served to define many of the bacterial genes involved in symbiosis with alfalfa. A genotype of Jemalong has been identified which can be regenerated after transformation byAgrobacterium, thus allowing the analysis ofin-vitro-modified genes in an homologous transgenic system. Finally, by virtue of the diploid, self-fertilising and genetically homogeneous character ofM. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant.

Keywords

symbiotic nitrogen fixationlegumeMedicago truncatula

Abbreviations

RFLP

restriction fragment length polymorphism

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • David G. Barker
    • 1
  • Sylvie Bianchi
    • 2
  • François Blondon
    • 3
  • Yvette Dattée
    • 2
  • Gérard Duc
    • 4
  • Sadi Essad
    • 5
  • Pascal Flament
    • 2
  • Philippe Gallusci
    • 1
  • Gérard Génier
    • 6
  • Pierre Guy
    • 6
  • Xavier Muel
    • 7
  • Jacques Tourneur
    • 7
  • Jean Dénarié
    • 1
  • Thierry Huguet
    • 1
  1. 1.Laboratoire de Biologie Moléculaire des Relations Plantes-MicroorganismesINRA-CNRSCastanet-Tolosan CedexFrance
  2. 2.Laboratoire d'Amélioration des PlantesUniversité de Paris-SudOrsay CedexFrance
  3. 3.Laboratoire du PhytotronCNRSGif sur YvetteFrance
  4. 4.Station de Génétique et d'Amélioration des PlantesINRA, BV 1540Dijon CedexFrance
  5. 5.Station de Génétique et d'Amélioration des PlantesINRAVersailles CedexFrance
  6. 6.Station d'Amélioration des Plantes FourragéresINRALusignanFrance
  7. 7.Laboratoire de Biologie CellulaireINRAVersailles CedexFrance
  8. 8.Max-Planck-Institut für ZüchtungsforschungAbteilung Genetische Grundlagen der PflanzenzüchtungKöln 30West Germany
  9. 9.Institut des Sciences VégétalesCNRSGif sur YvetteFrance