Plant Molecular Biology Reporter

, Volume 12, Issue 1, pp 37–42

An easy technique for the clearing of histochemically stained plant tissue

  • Tom Beeckman
  • Gilbert Engler
Protocol

DOI: 10.1007/BF02668662

Cite this article as:
Beeckman, T. & Engler, G. Plant Mol Biol Rep (1994) 12: 37. doi:10.1007/BF02668662

Abstract

The sites of GUS-activity in strongly pigmented whole-mount preparations or thick handmade sections of plant material can easily be visualized by clearing it with chlorallactophenol (CLP), without affecting the ClBr-indigo precipitate.

Key Words

GUShistochemical location

Abbreviations

GUS

β-glucuronidase

X-gluc

5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid

CLP

chlorallactophenol

CIBr-indigo

dichloro-dibromoindigo

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Tom Beeckman
    • 1
  • Gilbert Engler
    • 2
  1. 1.Laboratorium voor morfologie, systematiek en ecologie van de plantenUniversiteit GentGentBelgium
  2. 2.Laboratoire Associé de l’Institut National de la Recherche Agronomique (France)Universiteit GentGentBelgium