Amplification and sequencing of 16/18S rDNA from gel-purified total plant DNA
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- Bult, C., Källersjö, M. & Suh, Y. Plant Mol Biol Rep (1992) 10: 273. doi:10.1007/BF02668360
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We describe here methods and strategies for amplifying and sequencing the genes encoding the small subunits (16/18S) of nuclear and chloroplast ribosomal DNA (rDNA) from total plant DNA. These methods were developed in response to technical difficulties we encountered in our molecular systematic work with members of various plant families. These protocols have proved useful when the amount of tissue available for study is limited and when the tissues have high concentrations of undesirable secondary metabolites which are often co-isolated with nucleic acids.
Key wordsDNA extraction gel purification PCR amplification double-stranded DNA sequencing rDNA nuclear chloroplast