Glyceride composition of processed fats and oils as determined by glass capillary gas chromatography
- Cite this article as:
- D’Alonzo, R.P., Kozarek, W.J. & Wade, R.L. J Am Oil Chem Soc (1982) 59: 292. doi:10.1007/BF02662229
- 174 Downloads
Refined and bleached fats and oils can be analyzed directly by high-temperature glass capillary column gas chromatography after derivatization of the fatty acids, mono- and diglycerides with BSTFA [(N,O)-bis(trimethyl silyl) trifluoroacetamide]. The intact glycerides are separated on the basis of volatility to provide characteristic carbon number profiles (CNP). Quantitative information on mono-, di- and triglycerides, as well as free fatty acids, is obtained from a single, rapid separation. Profile values are reported for 13 different processed fats and oils. The analysis performed in the split-injection mode maintains column integrity after numerous separations, while producing acceptable relative standard deviations.