In Vitro

, Volume 20, Issue 7, pp 566–572

Lymphatic endothelial and smooth-muscle cells in tissue culture

  • M. G. Johnston
  • M. A. Walker

DOI: 10.1007/BF02639772

Cite this article as:
Johnston, M.G. & Walker, M.A. In Vitro (1984) 20: 566. doi:10.1007/BF02639772


Endothelial and smooth-muscle cells from bovine mesenteric lymphatic vessels have been collected and cultured in vitro. The endothelial cells grew as a monolayer exhibiting a “cobblestone” appearance with individual cells tending to be more flattened at confluence than their blood vascular counterparts. Approximately 30% of these cells expressed Factor VIII antigen compared with bovine mesenteric artery or human umbilical-vein endothelium in which the majority of cells were positive. The lymphatic smooth-muscle cells exhibited focal areas of multilayering and were Factor VIII negative. The availability of lymphatic endothelial and smooth-muscle cells in culture will provide a new tool for the investigation of the biological properties of the lymphatic vessels and their role in homeostasis.

Key words

lymphaticendotheliumsmooth muscletissue culture

Copyright information

© Tissue Culture Association, Inc 1984

Authors and Affiliations

  • M. G. Johnston
    • 1
  • M. A. Walker
    • 1
  1. 1.Department of Pathology, Medical Sciences BuildingUniversity of TorontoTorontoCanada