Lipids

, Volume 31, Issue 1, pp S23–S31

Dietary marine lipids suppress continuous expression of interleukin-1β gene transcription1

  • Dwight R. Robinson
  • Masaharu Urakaze
  • Riyun Huang
  • Hirofumi Taki
  • Eiji Sugiyama
  • Christopher T. Knoell
  • LiLian Xu
  • Edward T. H. Yeh
  • Philip E. Auron
The Role of Fatty Acids in Gene Expression

DOI: 10.1007/BF02637046

Cite this article as:
Robinson, D.R., Urakaze, M., Huang, R. et al. Lipids (1996) 31: S23. doi:10.1007/BF02637046

Abstract

n-3 Polyunsaturated fatty acids abundant in marine lipids suppress certain inflammatory and immune reactions, and dietary marine lipid supplements have antiinflammatory effects in experimental and human autoimmune disease. Previous work by other investigators demonstrated that dietary marine lipid supplements suppressed production of cytokines from stimulated human peripheral blood mononuclear cellsex vivo. The present study further documents the ability of n-3 fatty acids to inhibit cytokine formation, and in part defines the mechanism of the inhibition of production of interleukin-1β (IL-1β) by dietary n-3 fatty acid. Female BALB/c mice were each fed a fatfree balanced diet to which was added either a refined fish oil (FO) preparation as a source of n-3 fatty acid, or beef tallow (BT), which consisted primarily of saturated and monoenoic fatty acids. After ingesting the experimental diets for periods ranging from 3 to 12 wk, spleen cell preparations were stimulatedex vivo with either lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and prolL-1β mRNA (Il-1β mRNA) was measured by northern analysis. Levels of IL-1β mRNA in both LPS-and PMA-stimulated cells from BT-fed mice were elevated to a greater extent than in cells from FO-fed mice, at most concentrations of LPS and PMA. Stability of LPS-stimulated mRNA levels after actinomycin D was similar for BT and FO groups, indicating that lower levels of IL-1 mRNA with FO groups was related to suppressed IL-1 gene transcription and not due to accelerated transcript degradation. Nuclear run-on transcription assays revealed a more transient expression of the IL-1β gene in LPS-stimulated spleen cells from FO-fed mice compared to cells from BT-fed mice. We conclude that dietary marine lipids reduce transient expression of the IL-1β gene in stimulated splenic monocytic cells. Preliminary results from nuclear run-on transcription assays indicate that n-3 fatty acids may not change the initial rate of gene transcription but may promote more rapid shutting down of transcription of this gene after induction than do alternative lipids.

Abbreviations

BT

beef fallow

FO

lish oil

IL-1

interleukin-1

LPS

lipopolysaccharide

LT

leukotriene

PG

prostaglandin

PMA

phorbol 12-myristate 13-acetate

TNF-α

tumor necrosis factor-α

Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Dwight R. Robinson
    • 1
  • Masaharu Urakaze
    • 1
  • Riyun Huang
    • 1
  • Hirofumi Taki
    • 1
  • Eiji Sugiyama
    • 1
  • Christopher T. Knoell
    • 1
  • LiLian Xu
    • 1
  • Edward T. H. Yeh
    • 1
  • Philip E. Auron
    • 2
  1. 1.Arthritis Unit, Bulfinch 165Massachusetts General HospitalBoston
  2. 2.Department of Medicine, and the Center for Blood ResearchHarvard Medical SchoolMassachusettsBoston