DNA methylation and tissue culture-induced variation in plants

  • S. M. Kaeppler
  • R. L. Phillips
Somatic Cell Genetics/Genetic Transformation

DOI: 10.1007/BF02632283

Cite this article as:
Kaeppler, S.M. & Phillips, R.L. In Vitro Cell Dev Biol - Plant (1993) 29: 125. doi:10.1007/BF02632283


Plant cells growing in an artificial culture environment make numerous genetic mistakes. These alterations are manifested as increased frequencies of single-gene mutations, chromosome breakages, transposable element activations, quantitative trait variations, and modifications of normal DNA methylation patterns. Evidence is presented that indicates a high frequency of DNA hypomethylation as the result of the tissue culture process. Fifteen percent of the methylation changes appear to have been homozygous in the original regenerated plants. A hypothesis is advanced that relates DNA methylation to the variety of genetic alterations found among maize tissue culture regenerants and their progenies. The epigenetic nature of DNA methylation raises questions concerning the stability of tissue culture-induced changes in self-pollinations and crosses.

Key words

tissue culture corn DNA methylation somaclonal variation 

Copyright information

© Tissue Culture Association 1993

Authors and Affiliations

  • S. M. Kaeppler
    • 1
  • R. L. Phillips
    • 1
  1. 1.Department of Agronomy and Plant Genetics and Plant Molecular Genetics InstituteUniversity of MinnesotaSt. Paul
  2. 2.Department of AgronomyUniversity of NebraskaLincoln

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