A line of rat ovarian surface epithelium provides a continuous source of complex extracellular matrix Authors
Received: 12 March 1993 Accepted: 17 August 1993 DOI:
Cite this article as: Kruk, P.A. & Auersperg, N. In Vitro Cell Dev Biol - Animal (1994) 30: 217. doi:10.1007/BF02632043 Summary
A spontaneously immortalized, yet non-tumorigenic rat ovarian surface epithelial (ROSE 199) cell line, deposits large amounts of extracellular matrix (ECM) in response to crowding. The characteristics and components of ROSE 199-derived cell-free ECM were compared after three different preparative techniques: treatment with 20 m
M ammonium hydroxide, with 1% sodium deoxycholate, or by repeated freeze-thaws. The ECMs were analyzed by histochemistry, immunofluorescence, electron microscopy, and Western immunoblotting. Components of ROSE 199 ECM included laminin, fibronectin, and collagen types I and III. Even though ROSE 199 is an epithelial cell line, striated collagen fibers formed a major part of its matrix. Thus, ROSE 199 matrix consists of both basement membrane and stromal matrix components. This matrix supported the adhesion, spreading, and growth of several cell types without altering their morphology or growth pattern, and enhanced the attachment of some cell types that spread on plastic only with difficulty. Immunofluorescence, electron microscopy, and dry weight determinations indicated that a greater proportion of matrix was retained in preparations obtained by ammonium hydroxide or freeze thaw techniques than after sodium deoxycholate treatment. Ammonium hydroxide and freeze-thaw treated matrices were also superior to sodium deoxycholate preparations as evidenced by enhanced initial cellular adhesion and spreading compared to cells plated on plastic. Residual nuclear material did not seem to affect the biological activity of this matrix. ROSE 199 extracellular matrix provides a novel, complex substratum for cell culture and for studies of matrix functions and synthesis. Key words extracellular matrix ovarian surface epithelium Download to read the full article text References
Adams, A. T.; Auersperg, N. A cell line, ROSE 199, derived from normal rat ovarian surface epithelium. Exp. Cell Biol. 53:181–188; 1985.
Aggler, J. Three-dimensional organization of the extracellular matrix secreted by cultured rat smooth muscle cells. In Vitro Cell. Dev. Biol. 24:633–638; 1988.
Auersperg, N.; Hollinshead, A. C.; Lee, O. B., et al. Detection of herpes simplex virus tumor-associated antigens in human cell lines after long-term cultivation. In: Schlessinger, D., ed. Microbiology. Washington, DC: A.S.M. Press; 1981:302–307.
Auersperg, N.; MacLaren, I. A.; Kruk, P. A. Ovarian surface epithelium: autonomous production of connective tissue-type extracellular matrix. Biol. Reprod. 44:717–724; 1991.
Boyd, D.; Florent, G.; Chakrabarty, S., et al. Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material. Cancer Res. 48:2825–2831; 1988.
Brauer, P. R.; Keller, J. M. Ultrastructure of a model basement membrane lacking type IV collagen. Anat. Rec. 223:376–383; 1989.
Carley, W. W.; Milici, A. J.; Madri, J. A. Extracellular matrix specificity for the differentiation of capillary endothelial cells. Exp. Cell Res. 178:426–434; 1988.
Carlson, E. C.; Kenney, M. C. Preparation and histoarchitecture of ultrastructurally pure glomerular basement membrane. Renal Physiol. 3:280–287; 1980.
Chang, S.-G.; Toth, K.; Black, J. D., et al. Growth of human renal cortical tissue on collagen gel. In Vitro Cell. Dev. Biol. 28A:128–135; 1992.
Crickard, K.; Crickard, U.; Yoonessi, M. Human ovarian carcinoma cells maintained on extracellular matrix versus plastic. Cancer Res. 43:2762–2767; 1983.
Gillet, W. Artefactual loss of human ovarian surface epithelium: potential clinical significance. Reprod. Fertil. Dev. 3:93–98; 1991.
Gospodarowicz, D. Preparation of extracellular matrices produced by cultured bovine corneal endothelial cells and PF-HR-9 endodermal cells: their use in cell culture. In: Barnes, D. W.; Sirbaski, D. A.; Sato, G. H., eds. Methods for preparation of media, supplements, and substrata for serum-free animal cell culture. New York: Alan R. Liss; 1984:275–293.
Grinnell, F.; Fukamizu, F.; Pawelek, P., et al. Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in longterm cultures supplemented with ascorbic acid. Exp. Cell Res. 181:483–491; 1989.
Hadley, M. A.; Byers, S. W.; Suarez-Quian, C. A., et al. Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro. J. Cell. Biol. 101:1511–1522; 1985.
Hixon, D. C.; Ponce, M. D.; Allison, J. P., et al. Cell surface expression by adult rat hepatocytes of a non-collagen glycoprotein present in rat liver biomatrix. Exp. Cell Res. 152:402–424; 1984.
Hornby, A. E.; Pan, J.; Auersperg, N. Intermediate filaments in rat ovarian surface epithelial cells: changes with neoplastic progression in culture. Biochem. Cell Biol. 70:16–25; 1992.
Inoue, S.; Leblond, C. P.; Laurie, G. W. Ultrastructure of Reichert’s membrane, a multilayered basement membrane in the parietal wall of the rat yolk sac. J. Cell Biol. 97:1524–1537; 1983.
Kleinman, H. K.; Cannon, F. B.; Laurie, G. W., et al. Biological activities of laminin. J. Cell. Biochem. 27:317–325; 1985.
Kleinman, H. K.; McGarvey, M. L.; Hassell, J. R., et al. Basement membrane complexes with biological activity. Biochemistry 25:312–318; 1986.
Kopf-Maier, P.; Zimmermann, B. Organoid reorganization of human tumors under in vitro conditions. Cell Tissue Res. 264:563–576; 1991.
Kruk, P. A.; Auersperg, N. Human ovarian surface epithelial cells are capable of physically restructuring extracellular matrix. Am. J. Obstet. Gynecol. 167:1437–1443; 1992.
Laemmli, U. K. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685; 1970.
Leighton, J.; Tchao, R.; Nichols, J. Radial gradient culture on the inner surface of collagen tubes: organoid growth of normal rat bladder and rat bladder cancer cell line NBT-II. In Vitro Cell. Dev. Biol. 21:713–715; 1985.
Levine, A. E.; Black, B.; Brattain, M. G. Effects of
-dimethylformamide and extracellular matrix on transforming growth factor-
binding to a human colon carcinoma cell line. J. Cell. Physiol. 138:459–466; 1989.
Liotta, L. A.; Lee, C. W.; Morakis, D. J. New method for preparing large surfaces of intact human basement membrane for tumor invasion studies. Cancer Lett. 11:141–152; 1980.
Maines-Bandiera, S. L.; Kruk, P. A.; Auersperg, N. SV40 transformed human ovarian surface epithelial cells escape normal growth controls but retain morphogenetic responses to extracellular matrix. Am. J. Obstet. Gynecol. 167:729–735; 1992.
Massad, L. S.; Mutch, D. G.; Powell, C. B., et al. Expression of a resistance mechanism in ovarian and cervical carcinoma cells prevents their lysis by γ-interferon. Cancer Res. 50:4923–4928; 1990.
Meezen, E.; Hjelle, J. T.; Brendel, K. A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues. Life Sci. 17:1721–1732; 1975.
Milici, A. J.; Furie, M. B.; Carley, W. W. The formation of fenestrations and channels by capillary endothelium in vitro. Proc. Natl. Acad. Sci. USA 82:6181–6185; 1985.
Montesano, R. Cell-extracellular matrix interaction in morphogenesis: an in vitro approach. Experientia 42:977–985; 1986.
Morley, P.; Armstrong, D. T.; Gore-Langton, R. E. Fibronectin stimulates growth but not follicle-stimulating hormone-dependent differentiation of rat granulosa cells in vitro. J. Cell. Physiol. 132:226–236; 1987.
Nicosia, S. V.; Johnson, J. H. Surface morphology of the ovarian mesothelium (surface epithelium) and of other pelvic and extrapelvic mesothelial sites in the rabbit. Int. Soc. Gynecol. Pathol. 3:249–260; 1984.
Nicosia, S. V.; Johnson, J. H.; Steibel, E. J. Isolation and ultrastructure of rabbit ovarian mesothelium (surface epithelium). Int. J. Gynecol. Pathol. 3:348–360; 1984.
Nishida, T.; Ueda, A.; Fukuda, M., et al. Interactions of extracellular collagen and corneal fibroblasts: morphologic and biochemical changes of rabbit corneal cells cultured in a collagen matrix. In Vitro Cell. Dev. Biol. 24:1009–1014; 1988.
O’Guin, W. M.; Scherner, A.; Sun, T.-T. Immunofluorescence staining of keratin filaments in cultured epithelial cells. J. Tissue Cult. Methods 9:123–128; 1985.
Ossowski, L. In vivo invasion of modified chorioallantoic membrane by tumor cells: the role of cell surface-bound urokinase. J. Cell Biol. 107:2437–2445; 1988.
Rojkind, M.; Gatmaitan, Z.; Mackensen, S., et al. Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J. Cell Biol. 87:255–263; 1980.
Scott-Burden, T.; Resink, T. J.; Burgin, M., et al. Extracellular matrix: differential influence on growth and biosynthesis patterns of vascular smooth muscle cells from SHR and WKY rats. J. Cell. Physiol. 141:267–274; 1989.
Shimo-Oka, T.; Hasegawa, Y.; Ichio, I. Differential properties of attachment of human fibroblasts to various extracellular matrix proteins. Cell Struct. Funct. 13:515–524; 1988.
Siemens, C. H.; Auersperg, N. Serial propagation of human ovarian surface epithelium in tissue culture. J. Cell. Physiol. 134:347–356; 1988.
Simon-Assmann, P.; Bouziges, F.; Daviaud, D., et al. Synthesis of glycosaminoglycans by undifferentiated and differentiated HT29 human colonic cancer cells. Cancer Res. 47:4478–4484; 1987.
Tan, E.; Glassberg, E.; Orlsen, D. R., et al. Extracellular matrix gene expression by human endothelial and smooth muscle cells. Matrix 11:380–387; 1991.
Towbin, H.; Staehelin, T.; Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354; 1979.
Watt, F. The extracellular matrix and cell shape. T.I.B.S. 11:482–485; 1986.
Young, R. H.; Clement, P. B.; Scully, R. E. The ovary. In: Sternberg, S. S., ed. Diagnostic surgical pathology. New York: Raven Press; 1989:1655–1734.
Zubay, G. Biochemistry. Reading, MA: Addison-Wesley Publishing Co.; 1983:577–590.
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