In Vitro Cellular & Developmental Biology - Animal

, Volume 30, Issue 4, pp 209–216

Newly established cell lines fromDrosophila larval CNS express neural specific characteristics

  • Kumiko Ui
  • Shoko Nishihara
  • M. Sakuma
  • S. Togashi
  • R. Ueda
  • Y. Miyata
  • T. Miyake
Cellular Models

DOI: 10.1007/BF02632042

Cite this article as:
Ui, K., Nishihara, S., Sakuma, M. et al. In Vitro Cell Dev Biol - Animal (1994) 30: 209. doi:10.1007/BF02632042

Summary

From the central nervous system ofDrosophila melanogaster 3rd instar larvae, eight continuous cell lines have been established (named ML-DmBG1 to 8). Using ML-DmBG2, single colony isolation was carried out and six colonial clones were obtained. All reacted to the antibody to horseradish peroxidase, which is a neuronal marker in insects. Acetylcholine, a known neurotransmitter inDrosophila, was detected in three of the colonial clones by high performance liquid chromatography. Therefore, it is concluded that the established colonial clones are neural cells originating in the larval central nervous system. Among them, some variation was observed with respect to morphology, acetylcholine content, and reactivity to anti-HRP. The variation may reflect the heterogeneity of cells composing the central nervous system.

Key words

Drosophilacentral nervous systemcell linesanti-HRP antibodyacetylcholine

Copyright information

© Tissue Culture Association 1994

Authors and Affiliations

  • Kumiko Ui
    • 1
  • Shoko Nishihara
    • 1
  • M. Sakuma
    • 2
  • S. Togashi
    • 1
  • R. Ueda
    • 1
  • Y. Miyata
    • 2
  • T. Miyake
    • 1
  1. 1.Laboratory of Cell BiologyMitsubishi Kasei Institute of Life SciencesTokyoJapan
  2. 2.Department of PharmacologyNippon Medical SchoolTokyoJapan
  3. 3.Laboratory of Cell Biology, Institute of Life ScienceSoka UniversityTokyoJapan