In Vitro Cellular & Developmental Biology - Animal

, Volume 31, Issue 1, pp 14–24

Establishment and characterization of an immortalized but non-transformed human prostate epithelial cell line: BPH-1

Authors

  • S. W. Hayward
    • Department of AnatomyUniversity of California
  • R. Dahiya
    • Department of UrologyUniversity of California
  • G. R. Cunha
    • Department of AnatomyUniversity of California
  • J. Bartek
    • Danish Cancer SocietyDivision for Cancer Biology
  • N. Deshpande
    • Department of SurgeryLuton and Dunstable Hospital
  • P. Narayan
    • Department of UrologyUniversity of California
Cellular Models

DOI: 10.1007/BF02631333

Cite this article as:
Hayward, S.W., Dahiya, R., Cunha, G.R. et al. In Vitro Cell Dev Biol - Animal (1995) 31: 14. doi:10.1007/BF02631333

Summary

This report describes the development and characterization of an epithelial cell line (BPH-1) from human prostate tissue obtained by transurethral resection. Primary epithelial cell cultures were immortalized with SV40 large T antigen. One of the isolated clones was designated BPH-1. These cells have a cobblestone appearance in monolayer culture and are non-tumorigenic in nude mice following subcutaneous injection or subrenal capsule grafting. They express the SV40 large T antigen and exhibit increased levels of p53, as determined by immunocytochemistry. Cytogenetic analysis by G-banding demonstrated an aneuploid karyotype with a modal chromosome number of 76 (range 71 to 79,n=28) and 6 to 8 marker chromosomes. Some structurally rearranged chromosomes were observed, but the Y chromosome was normal. The expressed cytokeratin profile was consistent with a prostatic luminal epithelial cell. This profile was the same as that of primary prostatic epithelial cultures from which the BPH-1 cells were derived. In serum-free culture in plastic dishes epidermal growth factor (EGF), transforming growth factor (TGF)-α, fibroblast growth factor (FGF) 1 (aFGF), and FGF 7 (KGF) induced increased proliferation in these cells whereas FGF 2 (bFGF), TGF-β1, and TGF-β2 inhibited proliferative activity. Testosterone had no direct effect on the proliferative rate of BPH-1 cells. 5α-Reductase, 3α-hydroxysteroid oxidoreductase, and 17β-hydroxy-steroid oxidoreductase activities were detected in BPH-1 cells. Expression of androgen receptors and the secretory markers, prostate specific antigen and prostatic acid phosphatase, were not detectable by immunocytochemistry, biochemical assay, or RT-PCR analysis.

Key words

prostateepitheliumSV40growth factors

Copyright information

© Society for In Vitro Biology 1995