Detection of c- Sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization Authors
Received: 24 April 1991 Accepted: 09 September 1991 DOI:
Cite this article as: McClintock, J.T., Chan, I., Thaker, S.R. et al. In Vitro Cell Dev Biol - Animal (1992) 28: 102. doi:10.1007/BF02631013 Summary
Using in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-
cis proto-oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB 1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the production of PDGF-A and -B. Expression of both PDGF-A and -B transcripts were found to be localized predominantly in the cytoplasm of TGFB 1-treated RPE cells, with a portion of these cells displaying a hybridization response in the nuclear region. When compared to PMA- and thrombin-treated cells, TGFB 1 stimulated the RPE cell line to yield the greatest amount of detectable PDGF mRNA. In addition, the hybridization response observed in TGFB 1-treated cells was shown to be RNA dependent. Key words c- sis proto-oncogene mRNA detection horseradish peroxidase-labeled cDNA probes in situ hybridization Download to read the full article text References
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