In Vitro

, Volume 17, Issue 8, pp 713–718

Human smooth muscle cells cultured from atherosclerotic plaques and uninvolved vessel wall

  • S. G. Eskin
  • H. D. Sybers
  • J. W. Lester
  • L. T. Navarro
  • A. M. GottoJr.
  • M. E. DeBakey
Article

DOI: 10.1007/BF02628408

Cite this article as:
Eskin, S.G., Sybers, H.D., Lester, J.W. et al. In Vitro (1981) 17: 713. doi:10.1007/BF02628408

Summary

Smooth muscle cells (SMC) were cultured from atherosclerotic plaques and uninvolved arteries to determine if differences exist between growth characteristics or ultrastructure of the cultured cells. Eighteen aortic punch biopsies provided the uninvolved tissue, and 58 carotid plaques provided the atherosclerotic tissue. Eighty percent of the sample yielded viable cultured cells, which reached a maximum population doubling time during log phase growth of 72 h (seeding density=1.0×104 cells/cm2, 2nd passage). Growth characteristics of both normal and plaque-derived cells were the same in vitro. Growth rate declined with time in culture, and cell division ceased by the 5th or 6th passage. In culture, spindle shaped cells formed the “hill and valley” configuration typical of SMC. Plaquederived SMC were ultrastructurally similar to SMC from uninvolved vessel wall. Proliferative potential did not vary with age of sex, with method of culture, or with whether the cells were plaque derived or not.

Key words

smooth muscle cells atherosclerosis aging 

Copyright information

© Tissue Culture Association, Inc 1981

Authors and Affiliations

  • S. G. Eskin
    • 4
    • 1
    • 2
    • 3
  • H. D. Sybers
    • 4
    • 1
    • 2
    • 3
  • J. W. Lester
    • 4
    • 1
    • 2
    • 3
  • L. T. Navarro
    • 4
    • 1
    • 2
    • 3
  • A. M. GottoJr.
    • 4
    • 1
    • 2
    • 3
  • M. E. DeBakey
    • 4
    • 1
    • 2
    • 3
  1. 1.Department of PathologyBaylor College of MedicineHouston
  2. 2.Department of MedicineBaylor College of MedicineHouston
  3. 3.Department of PsychiatryBaylor College of MedicineHouston
  4. 4.Dept. of SurgeryBaylor College of MedicineHouston

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