In Vitro Cellular & Developmental Biology

, Volume 25, Issue 1, pp 44–48

A continuous alveolar macrophage cell line: Comparisons with freshly derived alveolar macrophages

  • Ronald J. Helmke
  • Victor F. German
  • John A. Mangos
Regular Papers

DOI: 10.1007/BF02624409

Cite this article as:
Helmke, R.J., German, V.F. & Mangos, J.A. In Vitro Cell Dev Biol (1989) 25: 44. doi:10.1007/BF02624409

Summary

Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonizedPseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.

Key words

macrophage alveolar in vitro phagocytosis oxidative burst 

Copyright information

© Tissue Culture Association, Inc 1989

Authors and Affiliations

  • Ronald J. Helmke
    • 1
  • Victor F. German
    • 1
  • John A. Mangos
    • 1
  1. 1.University of Texas Health Science Center at San AntonioSan Antonio

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