In Vitro Cellular & Developmental Biology

, Volume 25, Issue 7, pp 659–668

Expression of the human chondrocyte phenotype in vitro

  • Amy Lynn Aulthouse
  • Michael Beck
  • Edward Griffey
  • Julie Sanford
  • Karen Arden
  • Mirta A. Machado
  • William A. Horton
Regular Papers

DOI: 10.1007/BF02623638

Cite this article as:
Aulthouse, A.L., Beck, M., Griffey, E. et al. In Vitro Cell Dev Biol (1989) 25: 659. doi:10.1007/BF02623638

Summary

We report a culture scheme in which human epiphyseal chondrocytes lose their differentiated phenotype in monolayer and subsequently reexpress the phenotype in an agarose gel. The scheme is based on a method using rabbit chondrocytes. Culture in monolayer allowed small quantities of cells to be amplified and provided a starting point to study expression of the differentiated human chondrocyte phenotype. The cells cultured in monolayer produced type I procollagen, fibronectin, and small noncartilaginous proteoglycans. Subsequent culture in agarose was associated with the acquisition of typical chondrocyte ultrastructural features and the synthesis of type II collagen and cartilage-specific proteoglycans. The switch from the nonchondrocyte to the differented chondrocyte phenotype occurred under these conditions between 1 and 2 wk of agarose culture and was not necessarily homogeneous throughout a culture. This culture technique will facilitate direct investigation of human disorders of cartilage that have been addressed in the past by alternative approaches.

Key wordss

chondrogenesishuman chondrocyteagarose culturecollagenproteoglycans

Copyright information

© Tissue Culture Association, Inc 1989

Authors and Affiliations

  • Amy Lynn Aulthouse
    • 1
  • Michael Beck
    • 1
  • Edward Griffey
    • 1
  • Julie Sanford
    • 1
  • Karen Arden
    • 1
  • Mirta A. Machado
    • 1
  • William A. Horton
    • 1
  1. 1.Department of PediatricsUniversity of Texas Medical SchoolHouston
  2. 2.Department of Anatomical SciencesUniversity of Oklahoma, College of MedicineOklahoma City
  3. 3.Department of PediatricsUniversity of MainzMainzFRG