, Volume 24, Issue 3, pp 230-238

Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media

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Summary

We established for human fetal liver cells (cultured for 2 wk) in a hormonally defined medium, optimal conditions for attachment, multiplication, and preservation of epithelial morphology as well as production and secretion of serum proteins characteristic of fetal (α1-fetoprotein, AFP) and adult (albumin and hemopexin) life. Conditions were considered optimal when cell number, albumin, and hemopexin levels were maintained throughout the 2-wk culture period. However, the decrease in AFP concentration, which occurred after a few days of culture, could not be reversed. The culture system developed is a suitable model for studying regulatory mechanisms governing structure and function during differentiation and may prove useful for testing the effect of toxic agents during fetal development of the human liver.

This work was supported by the Medical Research Council of Canada (Grant MA-7768), a grant from the “Fonds de la recherche en sante du Quebec” (F.R.S.Q.) and by “CAFIR” funds from the University of Montreal. David W. Barnes is supported by NIH-NCI-grants 40475 and 01226. MS-P was recipient of a scholarship from the Notre-Dame Hospital Foundation and from the Universite de Montreal.J-FT received a summer studentship from the FRSQ and from the Canadian Liver Foundation.
Presented in preliminary form at the Tissue Culture Meeting in Houston, June 1984.