In Vitro Cellular & Developmental Biology

, Volume 21, Issue 7, pp 382–390

Establishment of a continuous cell line from fibrotic schistosomal granulomas in mice livers

  • Radovan Borojevic
  • Alvaro N. A. Monteiro
  • Solange A. Vinhas
  • Gilberto B. Domont
  • Paulo A. S. Mourão
  • Hervé Emonard
  • Gabriel GrimaldiJr.
  • Jean-Alexis Grimaud
Article

DOI: 10.1007/BF02623469

Cite this article as:
Borojevic, R., Monteiro, A.N.A., Vinhas, S.A. et al. In Vitro Cell Dev Biol (1985) 21: 382. doi:10.1007/BF02623469

Summary

A continuous murine cell line (GRX) was obtained from fibrotic granulomas induced in C3H/HeN mice liver by experimental infection withSchistosoma mansoni. This anchorage-dependent line produces composite connective tissue/extracellular matrix, displays morphological characteristics of myofibroblasts, and can, under appropriate conditions, accumulate fat droplets. GRX cells produce viral particles of retrovirus type. We consider GRX cell line to be representative of liver connective tissue cells, responsible for fibroplasia in liver fibrotic and granulomatous reactions.

Key words

liver fibrosisgranulomatous inflammationSchistosoma mansonimyofibroblastsIto cells

Copyright information

© Tissue Culture Association, Inc 1985

Authors and Affiliations

  • Radovan Borojevic
    • 1
    • 2
  • Alvaro N. A. Monteiro
    • 2
  • Solange A. Vinhas
    • 2
  • Gilberto B. Domont
    • 2
  • Paulo A. S. Mourão
    • 3
  • Hervé Emonard
    • 1
  • Gabriel GrimaldiJr.
    • 4
  • Jean-Alexis Grimaud
    • 2
  1. 1.Institut PasteurCNRS ERA 819LyonFrance
  2. 2.Departamento de Bioquimica, Instituto de QuimicaUniversidade Federal do Rio de JaneiroRio de JaneiroBrasil
  3. 3.Departamento de Bioquimica, Centro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrasil
  4. 4.Centro de Microscopia EletrônicaInstituto Oswaldo CruzRio de JaneiroBrasil