In Vitro Cellular & Developmental Biology

, Volume 22, Issue 6, pp 360–362

Comparison of trout hepatocyte culture on different substrates

  • Michael M. Lipsky
  • Talia R. Sheridan
  • Richard O. Bennett
  • Eric B. May
Rapid Communications in cell Biology

DOI: 10.1007/BF02623411

Cite this article as:
Lipsky, M.M., Sheridan, T.R., Bennett, R.O. et al. In Vitro Cell Dev Biol (1986) 22: 360. doi:10.1007/BF02623411

Summary

Comparisons were made of attachment and viability of rainbow trout (Salmo gairdneri) hepatocytes in short-term (2 days), primary culture on plastic, collagen-coated or extracellular matrix (ECM) coated dishes. Hepatocyte isolation routinely yielded cells with good viability (96%). Cells plated on ECM attached with high efficiency (93%) in contrast to cells cultured on plastic or collagen (∼20%). The cells plated on ECM flattened out and formed monolayers, while the cells on plastic and collagen rounded up and formed multi-cell aggregates in suspension. Viability of cells in all substrates remained high over the 2 day culture period. ECM is the first substrate to support trout-hepatocyte attachment in primary culture. Differentiated liver function was maintained in cells cultured on ECM as evidence by the induction of tyrosine aminotransferase by hydrocortisone (200%).

Key words

trout hepatocytes extracellular matrix culture substrate 

Copyright information

© Tissue Culture Association, Inc 1986

Authors and Affiliations

  • Michael M. Lipsky
    • 1
  • Talia R. Sheridan
    • 1
  • Richard O. Bennett
    • 1
  • Eric B. May
    • 1
  1. 1.Department of PathologyUniversity of Maryland School of MedicineBaltimore

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