Comparison of trout hepatocyte culture on different substrates
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Comparisons were made of attachment and viability of rainbow trout (Salmo gairdneri) hepatocytes in short-term (2 days), primary culture on plastic, collagen-coated or extracellular matrix (ECM) coated dishes. Hepatocyte isolation routinely yielded cells with good viability (96%). Cells plated on ECM attached with high efficiency (93%) in contrast to cells cultured on plastic or collagen (∼20%). The cells plated on ECM flattened out and formed monolayers, while the cells on plastic and collagen rounded up and formed multi-cell aggregates in suspension. Viability of cells in all substrates remained high over the 2 day culture period. ECM is the first substrate to support trout-hepatocyte attachment in primary culture. Differentiated liver function was maintained in cells cultured on ECM as evidence by the induction of tyrosine aminotransferase by hydrocortisone (200%).
- Bailey, G. S.; Taylor, M. J.; Selivonchick, D. P. Aflatoxin B1 metabolism and DNA binding in isolated hepatocytes from rainbow trout (Salmo gairdneri). Carcinogenesis 3:511–518; 1982. CrossRef
- Gospodarowicz, D.; Delgado, D.; Vlodavsky, I. Permissive effect of the extracellular matrix on cell proliferationin vitro. Proc. Natl. Acad. Sci. 77:4078–4094; 1980.
- Hayashi, S.; Oshiro, Z. Gluconeogenesis and glycolysis in isolated perfused liver of the cel, Bull. Jpn. Soc. Sci. Fish. 41:201–208; 1975.
- Hazel, J. R.; Prosser, C. L. Incorporation of I-14C-Acetate into fatty acids and sterols by isolated hepatocytes of thermally acclimated rainbow trout (Salmo gairdneri). J. Comp. Physiol. 134:321–329; 1979.
- Hendricks, J. D. Chemical carcinogenesis in fish. In: Weber, L. J., ed. Aquatic Toxicology. New York: Raven Press; 1982: 149–211.
- Hendricks, J. D.; Meyers, T. R.; Shelton, D. W.; Casteel, J. L.; Bailey, G. S. Hepatocarcinogenicity of benzo[a]pyrene to rainbow trout by dietary exposure and intraperitoneal injection. JNCI 74:839–847; 1985.
- Hsu, I. C.; Lipsky, M. M.; Cole, K. E.; Su, C. H.; Trump, B. F. Isolation and culture of hepatocytes from human liver of immediate autopsy. In Vitro Cellular Dev. Biol. 21:154–160; 1985. CrossRef
- Klaunig, J. E. Establishment of fish hepatocyte cultures for use inin vitro carcinogenicity studies. Natl. Cancer Inst. Mongr. 65:163–173; 1984.
- Klaunig, J. E.; Goldblatt, P. J.; Hinton, D. E.; Lipsky, M. M.; Chako, J.; Trump, B. F. Mouse liver cell culture. II. Primary culture. In Vitro 17:926–934; 1981. CrossRef
- Klaunig, J. E.; Ruch, R. J.; Goldblatt, P. J. Trout hepatocyte culture: isolation and primary culture. In Vitro Cellular Dev. Biol. 21:221–227; 1985. CrossRef
- Laishes, B. A.; Roberts, E.; Farber, E.In vitro measurement of carcinogen resistant liver cells during hepatocarcinogenesis. Int. J. Cancer. 21:186–193; 1978. CrossRef
- Lin, R. C.; Knox, W. E. Adaptation of the rat liver tyrosine α-ketoglutarate transaminase. Biochem. Biophys. Acta. 26:85–88; 1957. CrossRef
- Michalopoulos, G.; Pitot, H. C. Primary culture of parenchymal liver cells on collagen membranes. Exp. Cell Res. 94:70–78; 1975. CrossRef
- Parker, R. S.; Morrissey, M. T.; Moldeus, P.; Selivonchick, D. P. The use of isolated hepatocytes from rainbow trout (Salmo gardneri) in the metabolism of acetaminophen. Comp. Biochem. Physiol. 70B:631–633; 1981.
- Porthe-Nibelle, J.; Lahlow, B. Mechanisms of glucocorticoid uptake by isolated hepatocytes of the trout. Comp. Biochem. Physiol. 69B:425–433; 1981.
- Saez, L.; Golcoechea, O.; Anthauer, R.; Krauskopf, M. Behavior of RNA and protein synthesis during the acclimatization of the carp. Studies with isolated hepatocytes. Comp. Biochem. Physiol. 72B:31–38; 1982.
- Schoenhard, G. L.; Hendricks, J. D.; Nixon, J. E.; Lee, D. J.; Wales, J. H.; Sinnhuber, R. O.; Pawlowski, N. E. Aflatoxicol-induced hepatocellular carcinomas in rainbow trout (Salmo gairdneri) and the synergistic effects of cyclopropenoid fatty acids. Cancer Res. 41:1011–1014; 1981.
- Sinnhuber, R. O.; Hendricks, J. D.; Wales, J. H.; PutnamG.B. Neoplasms in rainbow trout, a sensitive animal model for environmental carcinogenesis. Ann. NY Acad. Sci. 298:389–408; 1977. CrossRef
- Wales, J. H.; Sinnhuber, R. O.; Hendricks, J. D.; Nixon, J. E.; Eisele, J. A. Aflatoxin B1 induction of hepatocellular carcinoma in the embryos of rainbow trout (Salmo gairdneri. JNCI 60:1133–1139; 1978.
- Walton, M. J.; Cowey, C. B. Gluconeogenesis by isolated hepatocytes from rainbow troutSalmo gairdneri. Comp. Biochem. Physiol. 62B:75–79; 1979.
- Whitham, M.; Nixon, J. E.; Sinnhuber, R. O. Liver DNA boundin vivo with aflatoxin B1 as a measure of hepatocarcinoma initiation in rainbow trout. JNCI 68:623–628; 1982.
- Williams, G. M.; Bermudez, E.; Scaramuzzino, D. Rat hepatocyte primary cell cultures. Improved dissociation and attachment technologies and the enhancement of survival by culture medium. In Vitro 13:809–817; 1978. CrossRef
- Comparison of trout hepatocyte culture on different substrates
In Vitro Cellular & Developmental Biology
Volume 22, Issue 6 , pp 360-362
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