Mechanical effects on endothelial cell morphology: In vitro assessment
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Endothelial cells are subjected to fluid mechanical forces which accompany blood flow. These cells become elongated and orient their long axes parallel to the direction of shear stress when the cultured cells are subjected to flow in an in vitro circulatory system. When the substrate is compliant and cyclically deformed, to simulate effects of pressure in the vasculature, the cells elongate an orient perpendicular to the axis of deformation. Cell shape changes are reflected in the alignment of microtubule networks. The systems described provide tools for assessing the individual roles of shear stress, pressure, and mechanical strain on vascular cell structure and function.
- Buck, R. C. Reorientation response of cells to repeated stretch and recoil of the substratum. Exp. Cell Res. 127:470–474; 1980. CrossRef
- Caro, C. G.; Fitzgerald, J. M.; Schroter, R. C. Atheroma and arterial wall shear. Proc. Soc. Lond. B177:109–159; 1971. CrossRef
- DeForrest, J. M.; Hollis, T. M. Shear stress and aortic histamine synthesis. Am. J. Physiol. 234:H701-H705; 1978.
- Dewey, C. F. Effects of fluid flow on living vascular cells. J. Biomech. Eng. 106:31–35; 1984. CrossRef
- Dewey, C. F.; Bussolari, S. R.; Gimbrone, M. A., et al. The dynamic response of vascular endothelial cells to fluid shear stress. J. Biomech. Eng. 103:177–185; 1981.
- Eskins, S. G.; Ives, C. L.; McIntire, L. V., et al. Response of cultured endothelial cells to steady flow. Microvasc. Res. 28:87–94; 1984. CrossRef
- Frangos, J. A.; Eskin, S. G.; McIntire, L. V., et al. Flow effects on prostacyclin production by cultured human endothelial cells. Science 227:1477–1479; 1985. CrossRef
- Franke, R. P.; Grafe, M.; Schnittler, H., et al. Induction of human vascular endothelial stress fibers by fluid shear stress. Nature 307:648–649; 1984. CrossRef
- Fry, D. L. Acute vascular endothelial changes associated with increased blood velocity. Circ. Res. 22:165–197; 1968.
- Gimbrone, M. A. Culture of vascular endothelium. Prog. Hemost. Thromb. 3:1–28; 1976.
- Ives, C. L.; Eskin, S. G.; McIntire, L. V., et al. The importance of cell origin and substrate in the kinetics of endothelial cell alignment in response to steady flow. Trans. Am. Soc. Art. Int. Org. 29:209–274; 1983.
- Langille, B. L.; Adamson, S. L. Relationship between blood flow direction and endothelial cell orientation at arterial branch sites in rabbits and mice. Circ. Res. 48:481–488; 1981.
- Leung, D. Y. M.; Glagov, S.; Mathews, M. B. A new in vitro system for studying cell response to mechanical stimulation. Exp. Cell Res. 109:285–298; 1977. CrossRef
- McIntire, L. V.; Eskin, S. G. Mechanical and biochemical aspects of leukocyte interaction with model vessel walls. In: Meiselman, H.; Lichtman, M.; LaCelle, P., eds. White cell mechanics. Alan R. Liss, Inc. New York, NY; 1984:209–219.
- Nerem, R. M.; Corhill, J. F. The role of fluid mechanics in atherogenesis. J. Biomech. Eng. 102:181–189; 1980. CrossRef
- Reidy, M. A.; Langille, B. L. The effect of local blood flow patterns on endothelial cell morphology. Exp. Mol. Pathol. 32:276–289; 1980. CrossRef
- Remuzzi, A.; Dewey, C. F.; Davies, P. F., et al. Orientation of endothelial cells in shear fields in vitro. Biorheology 21:617–630; 1984.
- Roach, M. R.; Smith N. B. Does high shear stress induced by blood flow lead to atherosclerosis? Perspect. Biol. Med. 26:287–303; 1983.
- Ross, R. Atherosclerosis: A problem of the biology of arterial wall cells and their interactions with blood components. Atherosclerosis 1:293–311; 1981.
- Ross, R.; Glomset, J. A. The pathogenesis of atherosclerosis. N. Engl. J. Med. 295:369–377; 420–425; 1976. CrossRef
- Sottiurai V. S.; Kollros, P.; Glagov, S., et al. Morphologic alteration of cultured arterial smooth muscle cells by cyclic stretching. J. Surg. Res. 35:490–497; 1983. CrossRef
- White, G. E.; Gimbrone, M. A.; Fujiwara, K. Factors influencing the expression of stress fibers in vascular endothelial cells in situ. J. Cell Biol. 97:416–424; 1983. CrossRef
- Wong, A. J.; Pollard, T. D.; Herman, I. M. Actin filament stress fibers in vascular endothelial cellsin vivo. Science 217:867–869; 1983. CrossRef
- Mechanical effects on endothelial cell morphology: In vitro assessment
In Vitro Cellular & Developmental Biology
Volume 22, Issue 9 , pp 500-507
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- endothelial cells
- shear stress
- Industry Sectors