Mechanical effects on endothelial cell morphology: In vitro assessment
Received: 25 July 1985 Accepted: 13 March 1986 DOI:
Cite this article as: Ives, C.L., Eskin, S.G. & McIntire, L.V. In Vitro Cell Dev Biol (1986) 22: 500. doi:10.1007/BF02621134 Summary
Endothelial cells are subjected to fluid mechanical forces which accompany blood flow. These cells become elongated and orient their long axes parallel to the direction of shear stress when the cultured cells are subjected to flow in an in vitro circulatory system. When the substrate is compliant and cyclically deformed, to simulate effects of pressure in the vasculature, the cells elongate an orient perpendicular to the axis of deformation. Cell shape changes are reflected in the alignment of microtubule networks. The systems described provide tools for assessing the individual roles of shear stress, pressure, and mechanical strain on vascular cell structure and function.
Key words endothelial cells compliance shear stress pressure alignment microtubules
This work was partially supported by grants HL 17437, HL 18072, and HL 23016 from the National Institutes of Health, Bethesda, MD, and grant C-938 from the Robert A. Welch Foundation.
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