In Vitro Cellular & Developmental Biology

, Volume 23, Issue 5, pp 349–354

Growth and hepatospecific gene expression of human hepatoma cells in a defined medium

  • G. J. Darlington
  • J. H. Kelly
  • G. J. Buffone
Article

DOI: 10.1007/BF02620991

Cite this article as:
Darlington, G.J., Kelly, J.H. & Buffone, G.J. In Vitro Cell Dev Biol (1987) 23: 349. doi:10.1007/BF02620991

Summary

The production of albumin, α-fetoprotein (AFP), and α-1 antitrypsin has been compared among human hepatoma cells cultured in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo. in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media. Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount of secreted albumin whereas insulin at 10 μg/ml reduced the level of total secreted protein.

Key words

hepatomaalbuminα-fetoproteinα1 antitrypsindefined medium

Copyright information

© Tissue Culture Association, Inc 1987

Authors and Affiliations

  • G. J. Darlington
    • 1
  • J. H. Kelly
    • 1
  • G. J. Buffone
    • 1
  1. 1.Department of Pathology, Baylor College of Medicine, One Baylor PlazaTexas Children's HospitalHouston