Article

In Vitro Cellular & Developmental Biology

, Volume 23, Issue 8, pp 546-558

First online:

In vitro modulation of antioxidant enzymes in normal and malignant renal epithelium

  • An Hang YangAffiliated withDepartment of Pathology, University of Wisconsin Medical School
  • , Terry D. OberleyAffiliated withDepartment of Pathology, University of Wisconsin Medical SchoolLaboratory Service, Electron Microscopy Section, William S. Middleton Memorial Veterans Hospital
  • , Larry W. OberleyAffiliated withRadiation Research Laboratory, University of Iowa College of Medicine
  • , Steven M. SchmidAffiliated withDepartment of Human Oncology, University of Wisconsin Medical School
  • , Kenneth B. CummingsAffiliated withDepartment of Human Oncology, University of Wisconsin Medical SchoolDepartment of Surgery, University of Wisconsin Medical School

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Summary

The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, were monitored in isolated human renal adenocarcinoma tissues and in cultured human renal adenocarcinoma cells. The results were compared to the activities of these enzymes in the proposed cell of origin, isolated human proximal tubular tissues, and cultured proximal tubular epithelial cells. Strong modulation of these enzymes by culture conditions was observed in normal cells but not in carcinoma cells. Low levels of cellular lipid peroxidation, as assessed by levels of malondialdehyde (MDA), were observed in adenocarcinoma cells under the culture conditions tested with one exception: greatly elevated MDA was observed in renal adenocarcinoma cells growth on plastic in serum-free, chemically defined medium. This increased lipid peroxidation correlated with a loss of cell viability under these conditions.

Key words

antioxidant enzymes renal epithelium