In Vitro Cellular & Developmental Biology

, Volume 23, Issue 8, pp 546–558

In vitro modulation of antioxidant enzymes in normal and malignant renal epithelium

Authors

  • An Hang Yang
    • Department of PathologyUniversity of Wisconsin Medical School
  • Terry D. Oberley
    • Department of PathologyUniversity of Wisconsin Medical School
    • Laboratory Service, Electron Microscopy SectionWilliam S. Middleton Memorial Veterans Hospital
  • Larry W. Oberley
    • Radiation Research LaboratoryUniversity of Iowa College of Medicine
  • Steven M. Schmid
    • Department of Human OncologyUniversity of Wisconsin Medical School
  • Kenneth B. Cummings
    • Department of Human OncologyUniversity of Wisconsin Medical School
    • Department of SurgeryUniversity of Wisconsin Medical School
Article

DOI: 10.1007/BF02620972

Cite this article as:
Yang, A.H., Oberley, T.D., Oberley, L.W. et al. In Vitro Cell Dev Biol (1987) 23: 546. doi:10.1007/BF02620972

Summary

The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, were monitored in isolated human renal adenocarcinoma tissues and in cultured human renal adenocarcinoma cells. The results were compared to the activities of these enzymes in the proposed cell of origin, isolated human proximal tubular tissues, and cultured proximal tubular epithelial cells. Strong modulation of these enzymes by culture conditions was observed in normal cells but not in carcinoma cells. Low levels of cellular lipid peroxidation, as assessed by levels of malondialdehyde (MDA), were observed in adenocarcinoma cells under the culture conditions tested with one exception: greatly elevated MDA was observed in renal adenocarcinoma cells growth on plastic in serum-free, chemically defined medium. This increased lipid peroxidation correlated with a loss of cell viability under these conditions.

Key words

antioxidant enzymesrenal epithelium

Copyright information

© Tissue Culture Association, Inc 1987