In Vitro Cellular amp; Developmental Biology

, Volume 21, Issue 10, pp 593–596

Human-human hybridomas secreting antibodies specific to human lung carcinoma

  • H. Murakami
  • S. Hashizume
  • H. Ohashi
  • K. Shinohara
  • K. Yasumoto
  • K. Nomoto
  • H. Omura
Rapid Communications in Cell Biology

DOI: 10.1007/BF02620891

Cite this article as:
Murakami, H., Hashizume, S., Ohashi, H. et al. In Vitro Cell Dev Biol (1985) 21: 593. doi:10.1007/BF02620891

Summary

Human Namalwa cells were screened in serum-free medium and in 6-thioguanine, then fused with human lymphocytes from lymph nodes of lung adenocarcinoma cancer patients. Extensive testing using 14 lung cancer cell lines, 11 other cancer cell lines and 4 normal fibroblast lines identified monoclonal antibodies produced by 4 hybridoma clones that reacted specifically with lung adenocarcinoma cells. These monoclonal antibodies also reacted with lung adenocarcinoma tissues and not normal tissues or erythrocytes of any blood type. These hybridoma clones grew and stably secreted the antibodies in serum-free medium as well as in serum-containing medium.

Key words

human hybridomaserum-free mediumlung adenocarcinoma

Copyright information

© Tissue Culture Association, Inc 1985

Authors and Affiliations

  • H. Murakami
    • 1
  • S. Hashizume
    • 2
  • H. Ohashi
    • 1
  • K. Shinohara
    • 1
  • K. Yasumoto
    • 3
  • K. Nomoto
    • 4
  • H. Omura
    • 1
  1. 1.Food Science and Technology InstituteKyushu UniversityFukuokaJapan
  2. 2.Morinaga Institute of Biological ScienceYokohamaJapan
  3. 3.Second SurgeryKyushu UniversityFukuokaJapan
  4. 4.Medical Institute of BioregulationKyushu UniversityFukuokaJapan