Symposia Proceeding Mechanisms of Cellular Control

In Vitro

, Volume 14, Issue 1, pp 155-161

First online:

Regulation of differentiation in normal and transformed erythroid cells

  • Richard A. RifkindAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Paul A. MarksAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Arthur BankAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Masaaiki TeradaAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Roberta C. ReubenAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , George M. ManiatisAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Eitan FibachAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Uri NudelAffiliated withCollege of Physicians and Surgeons, Columbia University
  • , Jane E. SalmonAffiliated withCollege of Physicians and Surgeons, Columbia University
    • , Yair GazittAffiliated withCollege of Physicians and Surgeons, Columbia University

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Summary

Studies are described employing two erythropoietic systems to elucidate regulatory mechanisms that control both normal erythropoiesis and erythroid differentiation of transformed hemopoietic precursors. Evidence is provided suggesting that normal erythroid cell precursors require erythropoietin as a growth factor that regulates the number of precursors capable of differentiating. Murine erythroleukemia cells proliferate without need of erythropoietin; they show a variable, generally low, rate of spontaneous differentiation and a brisk rate of erythropoiesis in response to a variety of chemical agents. Present studies suggest that these chemical inducers initiate a series of events including cell surface related changes, alterations in cell cycle kinetics, and modifications of chromatin and DNA structure which result in the irreversible commitment of these leukemia cells to erythroid differentiation and the synthesis of red-cell-specific products.

Key words

erythropoiesis Friend cell erythroleukemia erythropoietin differentiation