In Vitro

, Volume 14, Issue 6, pp 485–494

Gypsy moth cell lines divergent in viral susceptibility

I. Culture and identification
  • R. H. Goodwin
  • G. J. Tompkins
  • P. McCawley
Article

DOI: 10.1007/BF02616088

Cite this article as:
Goodwin, R.H., Tompkins, G.J. & McCawley, P. In Vitro (1978) 14: 485. doi:10.1007/BF02616088

Summary

A series of cell lines unique in insect virus susceptibility pattern have been isolated from the ovaries of the gypsy moth (Lymantria dispar: Lepidoptera: Lymantriidae) on a synthetic medium with mammalian and avian serum supplementation. Growth curves showed the poorest growth occurring on peptone-based media with somewhat better growth on amino-acid-based media. The best growth was obtained with combined media. Serological study distinguished the present cell lines from one another and from cell lines derived from other insect species grown routinely in the same laboratory. Baculovirus susceptibility among the new lines varied from no response to a specific complete replication response upon challenge by the homologous (gypsy moth) nuclear polyhedrosis virus.

Key words

insect cell culture serology culture media Lymantria (Porthetria) dispar Lepidoptera baculovirus 

Copyright information

© Tissue Culture Association 1978

Authors and Affiliations

  • R. H. Goodwin
    • 1
  • G. J. Tompkins
    • 1
  • P. McCawley
    • 1
  1. 1.Insect Pathology Laboratory, Plant Protection Institute, Agricultural Research ServiceU.S. Department of AgricultureBeltsville

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