Effects of cytosine arabinoside on in vivo and in vitro mouse limb development
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When pregnant mice were exposed to 40 mg per kg of cytosine arabinoside (ara-C) on days 10 to 12 of gestation, adactylous limbs with large, distally located blisters were found when the fetuses were examined on day 18. Embryonic limbs exposed transplacentally under identical conditions and explanted to culture exhibited the same morphological abnormality as did limbs exposed directly in culture to 0.1 to 1 μg per ml of ara-C. Two noncytotoxic analogues of ara-C, uridine arabinoside (ara-U) and hypoxanthine arabinoside (ara-HX), had no influence on morphological differentiation of limbs in vitro. Ara-C alone caused a dose-related decrease in uptake of3H-thymidine and35SO4 in cultured limb buds. Production of this morphologically distinct malformation in vitro will allow detailed biochemical investigations on the effect of ara-C limb ectodermal-mesenchymal interactions.
- Kochhar, D. M. 1970. Effects of azetidine-2-carboxylic acid, a proline analog, on chondrogenesis of cultured limb buds. In: R. Bass, F. Beck, H. J. Merker, D. Neubert, and R. Randhalin (Eds.),Metabolic Pathways in Mammalian Embryos During Organogenesis and its Modification by Drugs. Georg Thieme Publishers, Stuttgart, pp. 475–482.
- Kochhar, D. M., and T. K. Vest. 1974. Comparative effects of teratogenic agents on collagen biosynthesis in organ cultures of mouse limb buds (abstr). Third European Teratological Society Meeting, Helsinki.
- Kochhar, D. M., O. S. Kochhar, and S. M. Riebe. 1974. Electron microscopic observations on the developing chondrccytes in vitamin-A-treated mouse limb buds (abstr). American Association of Anatomy Meeting. Cleveland.
- Neubert, D., H. J. Merker, and S. Tapken 1974. Comparative studies on the prenatal development of mouse extremitiesin vivo and in organ culture. Naunyn-Schmiedeberg’s Arch. Pharmacol. 286a: 251–270. CrossRef
- Neubert, D., S. Tapkea, and H. J. Merker. Induction of skeletal malformations in organ cultures of mammalian embryonic tissues. Naunyn-Schmiedelberg’s Arch. Pharmacol. 286b: 271–282.
- Kochhar, D. M.. 1975. The use ofin vitro procedures in teratology. Teratology 11: 273–288. CrossRef
- Chou, T. C., D. J. Hutchison, F. A. Schmid, and F. S. Philips. 1975. Metabolism and selective effects of 1-B-D-arabinofuranosylcytosine in L1210 and host tissuesin vivo. Cancer Res. 35: 225–236.
- Momparler, R. L. 1974. A model for the chemotherapy of acute leukemia with 1-B-D-arabinofuranosylcytosine. Cancer Res. 34: 1775–1787.
- Chaube, S., W. Kreis, K. Uchida, and M. L. Murphy. 1968. The teratogenic effect of 1-B-D-arabinofuranosylcytosine in the rat. Biochem. Pharmacol. 17: 1213–1226. CrossRef
- Ritter, E. J., W. J. Scott, and J. G. Wilson, 1971. Teratogenesis and inhibition of DNA synthesis induced in rat embryos by cytosine arabinoside. Teratology 4: 7–14. CrossRef
- Scott, W. J., E. J. Ritter, and J. G. Wilson. 1975. Studies on induction of polydactyly in rats with cytosine arabinoside. Dev. Biol. 45: 103–111. CrossRef
- Krowke, R., W. Kiernan, and V. Rozkosny. 1975. Advances in the evaluation of drug effects on mammalian embryos performed with a biochemical screening system. In: D. Neubert, and H. J. Merker (Eds.),New Approaches to the Evaluation of Abnormal Embryonic Development. Georg Thieme Publishers, Stuttgart, pp. 614–645.
- Silage, S. 1965. Metabolism of 1-B-D-arabinofuranosylcytosine in L cells. Cancer Res. 25: 1446–1453.
- Miller, F. A., G. J. Dixon, J. Ehrligh, B. J. Sloan, and I. W. McLean. 1968. Antiviral activity of 9-B-D-arabinofuranosyladenine. In:Antimicrobial Agents and Chemotherapy. Academic, Press, New York pp. 136–148.
- Rugh, R. 1962.Experimental Embryology. Burgess, Minneapolis, p. 417.
- Aydelotte, M. B., and D. M. Kochhar. 1972. Development of mouse limb buds in organ culture. Chondrogenesis in the presence of a proline analog, L-azetidine-2-carboxylic acid. Dev. Biol. 28: 191–201. CrossRef
- Camiener, G. W., and C. G. Smith. 1965. Studies of the enzymatic deamination of cytosine arabinoside. I. Enzyme distribution and species specificity. Biochem. Pharmacol. 14: 1405–1416. CrossRef
- Milaire, J. 1965. Aspects of limb morphogenesis in mammals. In: R. DeHaan, and H. Ursprung (Eds.), Organogenesis. Holt, Rinehart and Winston, New York, pp. 283–300.
- Zwilling, E., and J. F. Ames. 1958. Polydactyly, related effects and axial shifts—a critique. Am. Nat. 92: 257–266. CrossRef
- Hamburg, M. 1952. Malformations in mouse erabryos induced by trypan blue. Nature 169(4288): 27. CrossRef
- Waddington, C. H., and T. C. Carter, 1953. A note on abnormalities induced in mouse embryos by trypan blue. J. Embryol. Exp. Morph. 1(2): 167–180.
- Mulherkar, L. 1960. The effects of trypan blue on chick embryos culturedin vitro. J. Embryol. exp. Morphol. 8(1): 1–5.
- Jurand, A. 1966. Early changes in limb buds of chick embryos after thalidomide treatment. J. Embryol. exp. Morphol. 16(2): 289–300.
- Corliss, C. E., A. A. Fedinec, and G. C. Robertson. 1966. The teratogenic effect of tetanus toxin on the central nervous system of the early chick embryo. Anat. Rec. 154(2): 221–232. CrossRef
- Effects of cytosine arabinoside on in vivo and in vitro mouse limb development
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