In Vitro

, Volume 13, Issue 4, pp 213–217

The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera; noctuidae)

  • J. L. Vaughn
  • R. H. Goodwin
  • G. J. Tompkins
  • P. McCawley
Article

DOI: 10.1007/BF02615077

Cite this article as:
Vaughn, J.L., Goodwin, R.H., Tompkins, G.J. et al. In Vitro (1977) 13: 213. doi:10.1007/BF02615077

Summary

The history and characteristics of two cell lines developed from primary explants of pupal tissue from the insect,Spodoptera frugiperda (J. E. Smith), are described. One cell line, IPLB-SF-21, was developed with hemolymph-supplemented medium and has been maintained continuously on the medium. The second cell line, IPLB-SF-1254, was developed with a medium containing a combination of vertebrate sera plus hemolymph and was adapted to hemolymph-free medium at the 6th passage. The IPLB-SF-1254 line is 36 hr. The chromosomal morphology and distribution was typical of other lepidopteran cell lines. Serological studies showed that both cell lines have at least one antigen which also is common to tissue antigens from pupae ofSpodoptera frugiperda.

Key words

characterization cell lines insect Spodoptera frugiperda Lepidoptera 

Copyright information

© Tissue Culture Association 1977

Authors and Affiliations

  • J. L. Vaughn
    • 1
  • R. H. Goodwin
    • 1
  • G. J. Tompkins
    • 1
  • P. McCawley
    • 1
  1. 1.Insect Pathology Laboratory, Plant Protection Institute, Agricultural Research ServiceU.S. Department of AgricultureBeltsville

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