Abstract
Various pectinolytic strains ofClostridium, Erwinia, andPseudomonas species produced methanol as a major end product during growth on pectin but not on glucose or polygalacturonic acid. Pectin metabolism ofClostridium butyricum strain 4P1 correlated with a final product concentration of 16 mM at the end of growth, and a 1:1 stoichiometry for methanol production and percent initial substrate methoxylation. Growth on pectin was associated with high activity of pectin methylesterase and the absence of methanol consumption. The ecological significance of pectin metabolism and the establishment of microbial methylotrophic metabolism in nature is discussed.
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Literature Cited
Colby, J., Dalton, H., Whittenbury, J. 1979. Biological and biochemical aspects of microbial growth on C1 compounds. Annual Review of Microbiology33:481–518.
Cox, R. B., Quayle, J. R. 1975. The autotrophic growth ofMicrococcus denitrificans on methanol. Biochemical Journal150:569–571.
Desikachary, T. V. 1959. Cyanophyta. New Delhi. Indian Council of Agricultural Research.
Donnelly, M. I., Dagley, S. 1980. Production of methanol from aromatic acids byPseudomonas putida. Journal of Bacteriology142:916–924.
Holdeman, L. V., Cato, E. P., Moore, W. E. C. 1977. Anaerobe laboratory manual, 4th ed. Blacksburg, Virginia: Virginia Polytechnic institute and State University.
Kamikubo, T., Hayashi, M., Nishio, N., Nagai, S. 1978. Utilization of nonsugar sources for vitamin B12 production. Applied and Environmental Microbiology35:971–973.
Kertesz, Z. I. 1951. The pectic substances. New York: Interscience.
Kirk, T. K., Connors, W. J., Zeikus, J. G. 1977. Advances in understanding the microbiological degradation of lignin, pp. 369–394. In: Loewus, F. A., Runeckles, V. C. (eds.), The structure, biosynthesis and degradation of wood. London, New York: Plenum.
Kuwahara, M. 1980. Metabolism of lignin-related compounds by bacteria, pp. 128–145. In: Kirk, T., Higuchi, T., Khang, Hn. (eds.) Lignin biodegradation: Microbiology, chemistry and potential applications. Boca Raton, Florida: CRC Press.
Prescott, G. W. 1973. The algae: A review. Boston: Houghton Mifflin.
Rexova-Benkova, L., Markovic, O. 1976. Pectic enzymes. Advances in Carbohydrate Chemistry33:323–385.
Schink, B., Zeikus, J. G. 1980. Role of microbial activity in wetwood, a new niche for anaerobic decomposition. Abstracts of the Annual Meeting of the American Society for Microbiology1980:102.
Somogyi, L. P., Romani, R. 1964. A simplified technique for the determination of pectin methylesterase activity. Analytical Biochemistry7:498–501.
Zeikus, J. G. 1980. Fate of lignin and related aromatics in anaerobic environments. pp. 101–110. In: Lignin biodegradation: Microbiology, chemistry and potential applications. Boca Raton, Florida: CRC Press.
Zeikus, J. G. 1977. The biology of methanogenic bacteria. Bacteriological Reviews41:514–541.
Zeikus, J. G., Ben-Bassat, A., Hegge, P. 1980. Microbiology of methanogenesis in thermal, volcanic environments. Journal of Bacteriology43:432–440.
Zeikus, J. G., Hegge, P. W., Anderson, M. A. 1979.Thermoanaerobium brockii gen. nov. and sp. nov., a new chemoorganotrophic, caldoactive anaerobic bacterium. Archives of Microbiology122:41–48.
Zeikus, J. G., Lynd, L. H., Thompson, T. E., Krzycki, J. A., Weimer, P. J., Hegge, P. W. 1980. Isolation and characterization of a new, methylotrophic, acidogenic anaerobe, the Marburg strain. Current Microbiology3:381–386.
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Schink, B., Zeikus, J.G. Microbial methanol formation: A major end product of pectin metabolism. Current Microbiology 4, 387–389 (1980). https://doi.org/10.1007/BF02605383
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DOI: https://doi.org/10.1007/BF02605383