Current Microbiology

, Volume 1, Issue 1, pp 19–23

Identification ofEscherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1-ELISA) procedure

Authors

  • Ann-Mari Svennerholm
    • Institute of Medical MicrobiologyUniversity of Göteborg
  • Jan Holmgren
    • Institute of Medical MicrobiologyUniversity of Göteborg
Article

DOI: 10.1007/BF02601701

Cite this article as:
Svennerholm, A. & Holmgren, J. Current Microbiology (1978) 1: 19. doi:10.1007/BF02601701

Abstract

A sensitive, quantitative method for determination ofEscherichia coli heat-labile enterotoxin (LT) is presented. The assay is based on the specific binding of LT to polystyrene-adsorbed GM1 ganglioside and subsequent enzyme immunological demonstration of the bound toxin. Enterotoxin titers determined with this GM1 enzyme-linked immunosorbent assay (ELISA) method agreed closely with those obtained with the adrenal cell bioassay. The GM1-ELISA procedure was capable of demonstrating LT in allE. coli overnight cultures that gave positive adrenal cell results. The simplicity and high reproducibility of the described method should make it well suited for routine laboratory diagnosis of LT enterotoxigenicE. coli strains.

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© Springer-Verlag New York Inc 1978