Originals

International Journal of Clinical and Laboratory Research

, Volume 24, Issue 2, pp 102-105

First online:

Comparison of four in vitro assays for specific IgE detection

  • Paola BoccagniAffiliated withInstitute of Immunology and Infectious Diseases, University of Verona
  • , Flavio FavariAffiliated withInstitute of Immunology and Infectious Diseases, University of Verona
  • , Giovanna ZanoniAffiliated withInstitute of Immunology and Infectious Diseases, University of Verona
  • , Angela PezziniAffiliated withInstitute of Immunology and Infectious Diseases, University of Verona
  • , Giuseppe TridenteAffiliated withInstitute of Immunology and Infectious Diseases, University of Verona

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Summary

Three immunoenzymatic techniques for specific IgE detection (Pharmacia CAP System, Kallestad Allercoat System, Neo Abellò Hamlet-IgE) and the classical Phadebas RAST were compared using 34 sera from patients with a clinical diagnosis of allergic disease and 19 sera from healthy non-atopic controls. IgE antibodies to 9 aeroallergens and 6 food antigens were assessed and 399 tests were run with each method. All techniques showed a high specificity (92%–100%) and satisfactory efficiency (82%–98%), while the sensitivity for RAST, CAP, Allercoat and Hamlet was 89%, 91%, 83% and 53%, respectively, with the lowest values for food allergens. There was a good overall correlation of the four techniques, except when the Hamlet method was compared with the other methods for food-specific IgE detection (correlation coefficient <0.3). These data indicate that CAP, Allercoat and RAST are satisfactory techniques for specific IgE determination, either for inhalants or for food allergens; CAP, however, offers the highest sensitivity without loss of specificity.

Key words

Allergy diagnosis Comparison of immunoenzymatic assays RAST Specific IgE