Interleukin-8 is constitutively and commonly produced by various human carcinoma cell lines

  • Kiyoshi Sakamoto
  • Takao Masuda
  • Seiji Mita
  • Taka-aki Ishiko
  • Yasunari Nakashima
  • Hirofumi Arakawa
  • Hiroshi Egami
  • Shinji Harada
  • Kouji Matsushima
  • Michio Ogawa
Originals

DOI: 10.1007/BF02591427

Cite this article as:
Sakamoto, K., Masuda, T., Mita, S. et al. Int J Clin Lab Res (1992) 22: 216. doi:10.1007/BF02591427

Summary

We examined the production of interleukin-8 and interleukin-6 by 30 human carcinoma cell lines. Serum levels of interleukin-8 were measured in 14 patients with hepatocellular carcinoma by enzyme-linked immunosorbent assay and Northern blotting. Furthermore, serum interleukin-8 was also investigated in a nude mouse bearing a tumor of the HuH7 hepatoma cell line producing interleukin-8. Of the 30 cell lines, 29 (96.7%) constitutively produced interleukin-8, and 19 of the 29 (65.5%) were high producers (>1 ng/ml culture supernatant). Among the high producers, 4 cell lines released both interleukin-8 and interleukin-6. Interleukin-6 was constitutively produced by 17 of the 30 (56.7%) cell lines, 4 of which (23.5%) were high producers (>1 ng/ml). By Northern blot analysis, mRNAs of interleukin-8 and interleukin-6 were detected in producing cell lines. Of 14 patients with hepatocellular carcinoma, 4 (28.5%) showed increased levels of serum interleukin-8. Furthermore, inoculation of the HuH7 hepatoma cell line which produced the highest amount of interleukin-8 into a nude mouse resulted in tumor production accompanied by an elevated level of human interleukin-8 (646 pg/ml) in the peripheral blood. Thus, interleukin-8 is constitutively and commonly produced by various carcinoma cell lines. The production of interleukin-8 by carcinoma cells may be related to the elevation of serum interleukin-8 in patients with hepatocellular carcinoma. Finally, these cell lines may be valuable for studying the relationship between interleukin-8 and cancer.

Key words

Interleukin-8 Cancer Cell lines 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Kiyoshi Sakamoto
    • 1
  • Takao Masuda
    • 2
  • Seiji Mita
    • 1
  • Taka-aki Ishiko
    • 1
  • Yasunari Nakashima
    • 1
  • Hirofumi Arakawa
    • 1
  • Hiroshi Egami
    • 1
  • Shinji Harada
    • 2
  • Kouji Matsushima
    • 3
  • Michio Ogawa
    • 1
  1. 1.Department of Surgery IIKumamoto University Medical SchoolKumamotoJapan
  2. 2.Department of Biodefence and Medical VirologyKumamoto University Medical SchoolKumamotoJapan
  3. 3.Department of Pharmacology, Cancer Research InstituteKanazawa UniversityKanazawaJapan

Personalised recommendations