Spontaneous firing of NG108-15 cells induced by transient exposure to ammonium chloride
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1. We report that NG108-15 (neuroblastoma × glioma) cells differentiated in defined serum-free media are capable of exhibiting stable automaticity (the spontaneous occurrence of regenerative action potentials) following exposure to extracellular perfusates containing NH4Cl.
2. Membrane depolarization (4–5 mV) concomitant with an increased pHi during NH4Cl exposure are followed by hyperpolarization (5–7 mV), subthreshold oscillations, and spontaneous firing after the removal of NH4Cl.
3. Cells cultured in 10% serum did not exhibit automaticity. Cells cultured in serum-free media are twice as likely to show automaticity as those cultured in reduced (1.5%) serum media.
4. We have examined factors that contribute to the events following NH4Cl exposure, namely, membrane depolarization and hyperpolarization, subthreshold oscillations, and automaticity. The inward currents activated at more negative potentials and the ionic currents associated with pronounced afterhyperpolarization in NG108-15 cells cultured in serum-free media provide a basis for the repetitive activity in general and automaticity in particular.
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- Spontaneous firing of NG108-15 cells induced by transient exposure to ammonium chloride
Cellular and Molecular Neurobiology
Volume 16, Issue 1 , pp 1-9
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- serum-free media
- ammonium chloride
- intracellular pH
- Industry Sectors
- Author Affiliations
- 1. Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Code 6900, 20375, Washington, DC
- 2. Department of Physiology, Uniformed Services University of the Health Sciences, 20814, Bethesda, MD
- 3. Electrophysics Branch, DPS/OST/CDRH, U.S. Food and Drug Administration, 20857, Rockville, MD
- 4. Section on Biophysics of Sensory Processes, LCB/NIDCD, National Institutes of Health, 20892, Bethesda, MD