, Volume 7, Issue 3, pp 223-228

Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions

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Abstract

Four methods for the determination of cell viability were compared: the plate count technique, the flow cytometer, and two microscopic numerations- one after methylene blue staining and the other one with epifluorescence. The experimental error of these techniques was for the first time estimated: 8% for both numerations under microscope and 13% for the plate count technique. The staining mechanisms were explained by comparing the numerations under microscope and the flow cytometer analysis.