Calcified Tissue International

, Volume 37, Issue 1, pp 25–31

Phagocytosis of bone collagen by osteoclasts in two cases of pycnodysostosis

  • Vincent Everts
  • Daniel C. Aronson
  • Wouter Beertsen
Clinical Investigations

DOI: 10.1007/BF02557674

Cite this article as:
Everts, V., Aronson, D.C. & Beertsen, W. Calcif Tissue Int (1985) 37: 25. doi:10.1007/BF02557674


Electron microscopic examination of bone biopsies obtained from two patients suffering from pycnodysostosis revealed that osteoclasts contained (sometimes large) cytoplasmic vacuoles filled with bone collagen fibrils. These vacuoles stained positive for acid phosphatase activity, thereby suggesting that bone matrix had been phagocytosed and subsequently exposed to hydrolytic enzymes of the lysosomal apparatus. Collagen-containing vacuoles were not observed in osteoclasts of individuals not suffering from this disease.

Key words

Osteoclasts Bone resorption Collagen degradation Phagocytosis Pycnodysostosis 

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Vincent Everts
    • 1
  • Daniel C. Aronson
    • 2
  • Wouter Beertsen
    • 3
  1. 1.Laboratory of Histology and Cell Biology, Faculty of MedicineUniversity of AmsterdamAZ AmsterdamThe Netherlands
  2. 2.Department of Pediatrics, Academic Medical CentreUniversity of AmsterdamAmsterdamThe Netherlands
  3. 3.Section of Experimental Oral Biology, Department of Periodontology, Faculty of DentistryUniversity of AmsterdamAmsterdamThe Netherlands

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