Calcified Tissue International

, Volume 45, Issue 3, pp 146–152

Localization of osteonectin expression in human fetal skeletal tissues byin situ hybridization

  • Marjo Metsäranta
  • Marian F. Young
  • Minna Sandberg
  • John Termine
  • Eero Vuorio
Clinical Investigations

DOI: 10.1007/BF02556057

Cite this article as:
Metsäranta, M., Young, M.F., Sandberg, M. et al. Calcif Tissue Int (1989) 45: 146. doi:10.1007/BF02556057

Summary

The expression of osteonectin gene was studied in developing human fetuses by Northern analysis andin situ hybridization. The highest levels of osteonectin mRNA were detected in RNA extracted from calvarial bones, growth plates, and skin. Low mRNA levels were present in several parenchymal tissues.In situ hybridization of developing long bones revealed three cell types with high osteonectin mRNA levels: osteoblasts, cells of the periosteum, and hypertrophic chondrocytes. Weaker signals were detected in osteocytes, fibroblasts of tendons, ligaments and skin, and in cells of the epidermis. Apart from the hypertrophic chondrocytes, only low osteonectin mRNA levels were seen in cartilage. The localization of osteonectin mRNA in fetal growth plates is consistent with the hypothesis that the protein plays a role in the mineralization of bone and cartilage matrices.

Key words

BoneCartilageOsteonectinCollagenSPARC

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Marjo Metsäranta
    • 1
  • Marian F. Young
    • 2
  • Minna Sandberg
    • 1
  • John Termine
    • 2
  • Eero Vuorio
    • 1
  1. 1.Department of Medical BiochemistryUniversity of TurkuTurkuFinland
  2. 2.Bone Research Branch, National Institutes of Dental ResearchNational Institutes of HealthBethesdaUSA
  3. 3.Department of Molecular GeneticsUniversity of Texas M. D. Anderson Cancer CenterHoustonUSA