Letters in Peptide Science

, Volume 9, Issue 4, pp 203–206

An accurate method for the quantitation of Fmoc-derivatized solid phase supports

Article

DOI: 10.1007/BF02538384

Cite this article as:
Gude, M., Ryf, J. & White, P.D. Lett Pept Sci (2002) 9: 203. doi:10.1007/BF02538384

Summary

By performing the Fmoc resin loading determination with DBU instead of piperidine, highly reproducible results were obtained that showed excellent correlation with data obtained by independent analytical methods.

Key words

Fmoc quantitationloading determinationresin substitution

Abbreviations

ACN

acetonitrile

Ams

O-amino-L-serine

BHA

benzhydrylamine functionalised polystyrene

Boc

t-butoxycarbonyl

DBF

dibenzofulvene

DBU

1,8-diazobicyclo[5.4.0]undec-7-ene

DCM

dichloromethane

DMF

dimethylformamide

Fmoc

fluoren-9-ylmethoxycarbonyl

HMPB

4-(4-hydroxymethyl-4-methoxyphenoxy)-butyryl

Me

methyl

Ph

phenyl

SPPS

solid phase peptide synthesis

TFA

trifluoroacetic acid

Trt

trityl

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Calbiochem-Novabiochem AGLäufelfingenSwitzerland
  2. 2.CN Biosciences (U.K.) LtdBeestonEngland