Effect of tocotrienols on the growth of a human breast cancer cell line in culture
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The tocotrienol-rich fraction (TRF) of palm oil consists of tocotrienols and some α-tocopherol (α-T). Tocotrienols are a form of vitamin E having an unsaturated side-chain, rather than the saturated side-chain of the more common tocopherols. Because palm oil has been shown not to promote chemically-induced mammary carcinogenesis, we tested effects of TRF and α-T on the proliferation, growth, and plating efficiency (PE) of MDA-MB-435 estrogen-receptor-negative human breast cancer cells. TRF inhibited the proliferation of these cells with a concentration required to inhibit cell proliferation by 50% of 180 μg/mL, whereas α-T had no effect at concentrations up to 1000 μg/mL as measured by incorporation of [3H]thymidine. The effects of TRF and α-T also were tested in longer-term growth experiments, using concentrations of 180 and 500 μg/mL. We found that TRF inhibited the growth of these cells by 50%, whereas α-T did not. Their effect on the ability of these cells to form colonies also was studied, and it was found that TRF inhibited PE, whereas α-T had no effect. These results suggest that the inhibition is due to the presence of tocotrienols in TRF rather than α-T.
- Sylvester, P.W., Russell, M., Ip, M.M., and Ip, C. (1986) Comparative Effects of Different Animal and Vegetable Fats Before and During Carcinogenesis Administration on Mammary Tumorigenesis, Sexual Maturation and Endocrine Function in Rats,Cancer Res. 46, 757–762.
- Sundram, K., Khor, H.T., Ong, A.S.H., and Pathmanathan, R. (1989), Effect of Dietary Palm Oils in Mammary Carcinogenesis in Female Rats Induced by 7,12-Dimethylbenz(α)anthracene,Cancer Res. 49, 1447–1451.
- Kritchevsky, D., Weber, M.M., and Klurfeld, D.M. (1992) Influence of Different Fats (soybean oil, palm olein or hydrogenated oil) on Chemically-Induced Mammary Tumors in Rats,Nutr. Res. 12, S175-S179. CrossRef
- Hubbard, N.E., and Erickson, K.L. (1987) Influence of Dietary Fats on Cell Populations of Line Mouse Mammary Tumors; A Morphometric and Ultrastructural Study,Cancer Lett. 35, 281–294. CrossRef
- Hopkins, G.J., and Carroll, K.K. (1979) Relationship Between Amount and Type of Dietary Fat in Promotion of Mammary Carcinogenesis Produced by 7,12-Dimethylbenz(α)anthracene,J. Natl. Cancer Inst. 62, 1009–1012.
- Clinton, S.K., Mulloy, A.L., and Visek, W.J. (1984) Effects of Dietary Saturation on Prolactin Secretion, Carcinogen Metabolism and Mammary Carcinogenesis in Rats,J. Nutr. 114, 1630–1639.
- Gammal, E.B., Carroll, K.K., and Plunkett, E.R. (1967) Effects of Dietary Fat on Mammary Carcinogenesis by 7,12-Dimethylbenz-α-anthracene in Rats,Cancer Res 27, 1737–1742.
- Rose, D.P., and Connolly, J.M. (1989) Stimulation of Growth of Human Breast Cancer Cell Lines in Culture by Linoleic Acid,Biochem. Biophys. Res. Comm. 164, 277–283. CrossRef
- Nesaretnam, K., Khor, H.T., Ganeson, J., Chong, Y.H., Sundram, K., and Gapor, A. (1992) The Effect of Vitamin E Tocotrienols from Palm Oil on Chemically Induced Mammary Carcinogenesis in Female Rats,Nutr. Res. 12, 879–892. CrossRef
- Tan, B. (1992) Antitumor Effects of Palm Carotenes and Tocotrienols in HRS/J Hairless Female Mice,Nutr. Res. 12, S163-S173. CrossRef
- Kato, A., Yamaoka, M., Tanaka, A., Komiyama, K., and Umezawa, I. (1985) Physiological Effect of Tocotrienols,Yakugaku Zasshi 34, 375–376.
- Komiyama, K., and Yamaoka, M. (1989) Studies on the Biological Activity of Tocotrienols,Chem. Pharm. Bull. 37, 1369–1371.
- Gould, M.N., Haag, J.D., Kennan, W.S., Tanner, M.A., and Elson, C.E. (1991) A Comparison of Tocopherol and Tocotrienol for the Chemoprevention of Chemically Induced Rat Mammary Tumors,Am. J. Clin. Nutr. 53, 1068s-1070s.
- Yamaoka, M., and Carrillo, M.J.H. (1990) Effect of Tocopherols and Tocotrienols on the Physicochemical Property of the Liposomal Membrane in Relation to Their Antioxidant Activity,Chem. Phys. Lipids 55, 295–300. CrossRef
- Serbinova, E., Kagan, V., Han, D., and Packer, L. (1991) Free Radical Recycling and Intermembrane Mobility in the Antioxidant Properties of α-Tocopherol and α-Tocotrienol,Free Radic. Biol. Med. 10, 263–275. CrossRef
- Lathia, D., Braasch, A., and Theissen, U. (1988) Inhibitor Effects of Vitamin C and E onIn Vitro Formation ofN-Nitrosamine Under Physiological Conditions,Front. Gastrointest. Res. 14, 151–156.
- Salomen, J.T., Salomen, R., and Lappetalainen, R. (1985) Risk of Cancer in Relation to Serum Concentrations of Selenium and Vitamin A and E, Match Case Control Analysis of Prospective Data,Br. Med. J. 290, 417–420. CrossRef
- Willet, W.C. (1986) Selenium, Vitamin E, Fiber and the Incidence of Human Cancer an Epidemiologic Perspective,Adv. Exp. Med. Biol. 206, 27–34.
- Knekt, P., Aromaa, A., Maatela, J., Aaran, R.K., Nikkari, T., Hakama, M., Hakulinen, T., Peto, R., Saxen, E., and Teppo, L. (1988) Serum Vitamin E and Risk of Cancer Among Finnish Men During a Ten Year Follow-Up,Am. J. Epidemiol. 127, 28–41.
- Knekt, P. (1988) Serum Vitamin E Level and Risk of Female Cancer,Int. J. Epidemiol. 17, 281–286.
- Packer, L. (1991) Protective Role of Vitamin E in Biological System,Am. J. Clin. Nutr. 53, 1050S-1055S.
- Sundram, K., and Gapor, A. (1992) A Vitamin E from Palm Oil Its Extraction and Nutritional Properties,Lipid Technolog., November/December, 137–141.
- Price, J.E., Polyzos, A., Zhang, R.D., and Daniels, L.M. (1990) Tumorigenicity and Metastasis of Human Breast Carcinoma Cell Lines in Nude Mice,Cancer Res. 50, 717–721.
- Hansen, M.B., Nielsen, S.E., and Burg, K. (1989) Re-Examination and Further Development of a Precise and Rapid Dye Method for Measuring Cell Growth/Cell Kill,J. Immunol. Methods 119, 203–210. CrossRef
- Leth, T., and Sondergaard, H. (1977) Biological Activity of Vitamin E Compounds and Natural Materials by the Resorption-Gestation Test and Chemical Determination of the Vitamin E Activity in Foods and Feeds,J Nutr. 107, 2236–2243.
- Wattenberg, L.W. (1972) Inhibition of Carcinogenic and Toxic Effects of Polycyclic Hydrocarbons by Phenolic Antioxidants and Ethoxyquin,J. Natl. Cancer Inst. 48, 1425–1430.
- Dayton, S., Hashimoto, S., and Wollman, J. (1977) Effect of High-Oleic and High Linoleic Safflower Oils on Mammary Tumors Induced in Rats by 7,12-Dimethylbenzanthracene,J. Nutr. 107, 1353–1360.
- Welsch, C.W. (1992) Relationship Between Dietary Fat and Experimental Mammary Tumorigenesis: A Review and Critique,Cancer Res. 52, 2040s-2048s.
- Sumiyoshi, H. (1985) Effects of Vitamin E Deficiency on 1,2 Dimethylhydrazine Induced Intestinal Carcinogenesis in Rats,Hiroshima J. Med. Sci. 34, 363–369.
- Ip, C. (1982) Dietary Vitamin E Intake and Mammary Carcinogenesis in Rats,Carcinogenesis 3, 1453–1456.
- Horvath, P.M. and Ip, C. (1983) Synergistic Effect of Vitamin E and Selenium in the Chemoprevention of Mammary Carcinogenesis in Rats,Cancer Res. 43, 5335–5341.
- Ngah, W.Z.W., Jarien, Z., San, M.M., Marzuki, A., Top, G.M., Shamaan, N.A., and Kadir, K.A. (1991) Effects of Tocotrienols on Hepatocarcinogenesis Induced by 2 Acetylaminofluorene in Rats,Am. J. Clin. Nutr. 53, 1076s-1081s.
- Effect of tocotrienols on the growth of a human breast cancer cell line in culture
Volume 30, Issue 12 , pp 1139-1143
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Palm Oil Research Institute of Malaysia, P.O. Box 10260, 50720, Kuala Lumpur, Malaysia
- 2. University of Western Ontario, N6A 5CI, London, Ontario
- 3. London Regional Cancer Research Centre, N6A 4L6, London, Ontario, Canada