Lipids

, Volume 27, Issue 4, pp 270–274

Oxysterols in cultured bovine aortic smoth muscle cells and in the monocyte-like cell line U937

  • Jae Eun Pie
  • Christine Seillan
Article

DOI: 10.1007/BF02536474

Cite this article as:
Pie, J.E. & Seillan, C. Lipids (1992) 27: 270. doi:10.1007/BF02536474

Abstract

Oxysterols in cultured bovine aortic smooth muscle cells and in the monocyte-like cell line U937 were analyzed by gas-liquid chromatography. The following products were detected: 7α- and 7β-hydroxycholesterol, 7-ketocholesterol, cholesterol-α-epoxide, cholesterol-β-epoxide, cholestanetriol, and 20- and 25-hydroxycholesterol. The proportions of oxidized cholesterol varied from 1 to 3% of total cholesterol. The concentrations of oxysterols were lower when the smooth muscle cells were maintained in a vitamin E-enriched medium, and were higher in U937 cells when the cells were activated with phorbol myristate acetate. The cell oxysterol content appears to be regulated by factors that inhibit or enhanced free radical generation. The concentrations of oxysterols found in cells may serve as an indicator of the extent of lipid peroxidation.

Abbreviations

BHT

butylated hydroxytoluene

GLC

gas-liquid chromatography

HEPES

N-(2-hydroxyethyl)-piperazine-N′-(2-ethanesulfonic acid)

PMA

phorbol 12-myristate 13-acetate

TLC

thin-layer chromatography

Nomenclature

Cholestanetriol

cholestane-3β,5α,6β-triol

cholestanol

5α-cholestan-3β-ol

cholesterol

cholest-5-en-3β-ol

cholesterol-α-epoxide

5,6α-epoxy-5α-cholestan-3β-ol

cholesterol-β-epoxide

5,6β-epoxy-5α-cholestan-3β-ol

7α-hydroxycholesterol

cholest-5-ene-3β,7α-diol

7β-hydroxycholesterol

cholest-5-ene-3β,7β-diol

19-hydroxycholesterol

cholest-5-ene-3β,19-diol

20-hydroxycholesterol

cholest-5-ene3β,20α-diol

25-hydroxycholesterol

cholest-5-ene-3β,25-diol

26-hydroxycholesterol

cholest-5-ene-3β, 26-diol

7-ketocholesterol

3β-hydroxycholest-5-en-7-one

Copyright information

© American Oil Chemists’ Society 1992

Authors and Affiliations

  • Jae Eun Pie
    • 1
  • Christine Seillan
    • 1
  1. 1.Laboratoire de Nutrition et Sécurité AlimentaireINRA-CRJJouy-en-Josas CédexFrance

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