Lipids

, Volume 26, Issue 8, pp 619–627

Metabolism of plant sterols by nematodes

  • David J. Chitwood
  • William R. Lusby
Plant and Fungal Sterols: Biosynthesis, Metabolism and Function Papers Presented at a Symposium Held at the AOCS Annual Meeting. Baltimore, MD, April 1990 Review

DOI: 10.1007/BF02536426

Cite this article as:
Chitwood, D.J. & Lusby, W.R. Lipids (1991) 26: 619. doi:10.1007/BF02536426

Abstract

Parasitic nematodes do not biosynthesize sterolsde novo and therefore possess a nutritional requirement for sterol, which must be obtained from their hosts. Consequently, the metabolism of phytosterols by plant-parasitic nematodes is an important process with potential for selective exploitation. The sterol compositions of several species of plant-parasitic nematodes were determined by capillary gas chromatography-mass spectrometry and compared with the sterol compositions of their hosts. Saturation of the phytosterol nucleus was the major metabolic transformation performed by the root-knot nematodesMeloidogyne arenaria andM. incognita and the corn root lesion nematode,Prytalenchus agilis. In addition to saturation, the corn cyst nematode,Heterodera zeae, dealkylated its host sterols at C-24. Because free-living nematodes can be cultured in sterol-defined artificial medium, they have been successfully used as model organisms for investigation of sterol metabolism in plant-parasitic nematodes. Major pathways of phytosterol metabolism inCaenorhabditis elegans, Turbatrix aceti andPanagrellus redivivus incleded C-24 dealkylation and 4α-methylation (a pathway unique to nematodes).C. elegans andT. aceti introduced double bonds at C-7, andT. aceti andP. redivivus saturated the sterol nucleus similarly to the plant-parasitic species examined. Several azasteroids and long-chain dimethylalkylamines inhibited growth and development ofC. elegans and also the Δ24-sterol reductase enzyme system involved in the nematode C-24 dealkylation pathway.

Abbreviations

GC-MS

gas chromatography-mass spectrometry

GLC

gas-liquid chromatography

NMR

nuclear magnetic resonance

RRT

relative retention time

UV

ultraviolet spectroscopy

Copyright information

© American Oil Chemists’ Society 1991

Authors and Affiliations

  • David J. Chitwood
    • 1
  • William R. Lusby
    • 1
  1. 1.Nematology Laboratory and Insect Neurobiology and Hormone LaboratoryUSDA-ARSBeltsville

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