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Rapid determination of double bond configuration and position along the hydrocarbon chain in cyclic fatty acid monomers

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Lipids

Abstract

This study reports the structural elucidation of diunsaturated 5- or 6-membered ring cyclic fatty acid monomers (CFAM) isolated from heated flaxseed oil by complementary gas chromatography (GC)-mass spectrometry (MS) and GC-matrix isolation-Fourier transform infrared spectroscopy (MI-FTIR). Infrared measurements of CFAM were carried out on methyl ester derivatives as well-resolved chromatograms were obtained on a polar 100% cyanopropyl polysiloxane capillary GC column. By contrast, electron ionization MS of methyl ester derivatives was of limited value because of double bond migration during the ionization process in the mass spectrometer. This communication reports definitive MS fragmentation patterns that can confirm ring position and double bond position along the fatty acid chain in 1,2-disubstituted CFAM determined as 2-alkenyl-4,4-dimethyloxazoline derivatives. Double bond configuration (cis, trans, or conjugatedcis,cis) in CFAM was confirmed by GC-MI-FTIR. The presence of CFAM, degradation products found in used frying oils, is a potential source of dietary toxicity.

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Abbreviations

AMP:

2-amino-2-methyl-1-propanol

CFAM:

cyclic fatty acid monomers

EI:

electron ionization

FAME:

fatty acid methyl esters

FTIR:

Fourier transform infrared spectroscopy

GC:

gas chromatography

MI:

matrix isolation

MS:

mass spectrometry

PFK:

perfluorokerosence

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Authors and Affiliations

  1. Food and Drug Administration, National Center for Food Safety and Technology, 60501, Summit-Argo, Illinois

    R. E. McDonald

  2. University of Illinois, 61801, Urbana, Illinois

    B. D. Flickinger & E. G. Perkins

  3. Center for Food Safety and Applied Nutrition, Food and Drug Administration (HFS-717), 20204, Washington, DC

    M. M. Mossoba, M. P. Yurawecz, J. A. G. Roach & H. S. Lin

Authors
  1. M. M. Mossoba
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  2. M. P. Yurawecz
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  3. J. A. G. Roach
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  4. H. S. Lin
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  5. R. E. McDonald
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  6. B. D. Flickinger
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  7. E. G. Perkins
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Mossoba, M.M., Yurawecz, M.P., Roach, J.A.G. et al. Rapid determination of double bond configuration and position along the hydrocarbon chain in cyclic fatty acid monomers. Lipids 29, 893–896 (1994). https://doi.org/10.1007/BF02536259

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  • DOI: https://doi.org/10.1007/BF02536259

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