Arachidonic acid biosynthesis in non-stimulated and adrenocorticotropin-stimulated sertoli and leydig cells
- Cite this article as:
- Hurtado de Catalfo, G.E., Mandon, E.C. & de Gómez Dumm, I.N.T. Lipids (1992) 27: 593. doi:10.1007/BF02536116
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The biosynthesis of arachidonic acid in Sertoli and Leydig cells isolated from the testes of mature rats has been investigated. Both types of cells incorporated [2-14C]eicosatrienoic acid from the incubation medium and transformed it into arachidonic acid. The administration of adrenocorticotropin (ACTH) to the rats decreased the Δ5 desaturating activity in the isolated testicular cells, while ACTH produced no changes in the uptake of the substrate. Similar results were obtained when ACTH was added to the incubation medium of cells isolated from non-hormone treated rats. The total fatty acid composition of the Sertoli cells isolated from ACTH-treated rats showed a significant increase in the relative percentage of 18∶2n−6 and a decrease in the C20 and C22 polyenes. This may indicate that ACTH exerts an inhibitory effect on Δ6, Δ5 and Δ4 desaturase activities. Addition of corticosterone to the incubation medium also produced a significant decrease in arachidonic acid biosynthesis. Because ACTH is known to stimulate the release of corticosteronein vivo, both hormones may act cumulatively in the regulation of arachidonic acid metabolism in Sertoli and Leydig cells.
cyclic adenosine monophosphate
Krebs Ringer bicarbonate glucose
polyunsaturated fatty acids