, Volume 24, Issue 10, pp 873–881

Thiobarbituric acid-reactive substances from peroxidized lipids

  • Hiroko Kosugi
  • Takashi Kojima
  • Kiyomi Kikugawa

DOI: 10.1007/BF02535762

Cite this article as:
Kosugi, H., Kojima, T. & Kikugawa, K. Lipids (1989) 24: 873. doi:10.1007/BF02535762


The thiobarbituric acid (TBA) reaction was performed on linoleic acid 13-monohydroperoxide, autoxidized fatty esters, edible fats and oils, rat liver microsomal lipids, and on human erythrocyte ghost lipids in order to determine which substances from peroxidized lipids are TBA-reactive. The reaction was carried out in 2% acetic acid containing butylated hydroxytoluene using two different reaction modes: a one-step mode which involves heating at 100°C, and a two-step mode which involves first treatment at 5°C and subsequent heating at 100°C. Yields of the red 1∶2 malonaldehyde/TBA adduct, as estimated by absorbance, fluorescence intensity and high-performance liquid chromatography, were much higher than the malonaldehyde content as determined by direct chemical analysis. Yields of red pigment obtained by the two-step mode were slightly higher than those obtained by the one-step mode. Pigment yields were dramatically increased by addition oft-butyl hydroperoxide. Red pigment formation from alkenals and alkadienals was similarly enhanced by the two-step mode or by addition oft-butyl hydroperoxide, whereas pigment formation from malonaldehyde was not. It appears likely that a component of the total red pigment formed from the peroxidized lipids was due to aldehyde species other than malonaldehyde.



thiobarbituric acid


t-butyl hydroperoxide




butylated hydroxytoluene


active oxygen method


highperformance liquid chromatography

Copyright information

© American Oil Chemists’ Society 1989

Authors and Affiliations

  • Hiroko Kosugi
    • 1
  • Takashi Kojima
    • 2
  • Kiyomi Kikugawa
    • 2
  1. 1.Ferris Women's CollegeIzumiku, YokohamaJapan
  2. 2.Tokyo College of PharmacyHachioji TokyoJapan